Medtronic, Inc.

Restoragen, Inc.

Nps Pharmaceuticals, Inc.

Evolve Biologics Inc.

Jin Seog Seo

Daniel J Strydom

Barton Holmquist

Fred W Wagner

Peng Luan

Yuannan Xia

Mary J Bossard

Edwin H Merrifield

David F Miller

Kirk Cryer

A method for the purification of albumin from plasma is described. The method comprises (a) contacting the plasma with sodium caprylate (NaCP) at an amount dependent on total protein concentration and the ratio of NaCP to total protein in the plasma, (b) heating the plasma at a near neutral pH range, and (c) separating the albumin from non-albumin-phase. The said method provides for a high yield and purity albumin solution.

Method for purification of albumin

A palladate palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladate palladium promoter dissolved in a high-concentration acidic organic acid solvent.

Polypeptide cleavage process

The invention provides a process for amidating a desired peptide comprising cleaving a substrate polypeptide at a X-cysteine sequence, wherein Xis the amino acid at the peptide carboxyl-terminus and cysteine is the first amino acid of a palladium cleavage site comprising the sequence cysteine-X-X, wherein Xis any amino acid, Xis an amino acid selected from the group consisting of cysteine, histidine, or methionine, and wherein the carboxyl-terminus of the peptide is amidated upon cleavage at the X-cysteine sequence.

Composition for palladium-mediated cleavage of peptides containing CXC, CXH or CHM sequences

The invention provides methods for making peptides from a polypeptide containing at least one copy of the peptide using clostripain to excise the peptide from the polypeptide. The methods enable the use of a single, highly efficient enzymatic cleavage to produce any desired peptide sequence.

Method for enzymatic production of GLP-2(1-33) and GLP-2(1-34) peptides

A palladatepalladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladatepalladium promoter dissolved in a high-concentration acidic organic acid solvent.

The invention provides a palladium-promoted hydrolytic polypeptide cleavage process which selectively cleaves the polypeptide at a Cys-His cleavage site comprising solubilizing the polypeptide in a reaction mixture comprised of a palladium promoter dissolved in a high-concentration formic acid, wherein the concentration of the formic acid in the reaction mixture is between about 1 to about 22 molar.

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Brampton, Canada

Jin Seog Seo