Zymogenetics, Inc.

Jin-Jyi Chang

Paul D Bishop

Gerald W Lasser

John W Forstrom

William Downey

Mads Laustsen

Andrew R Alaska

Linh Phan

Methods of removing protein contaminants from a solution comprising thrombopoietin are disclosed. The solution is exposed to hydroxyapatite, whereby protein contaminants are bound to the hydroxyapatite and the thrombopoietin remains substantially unbound, and the unbound thrombopoietin is recovered. The use of hydroxyapatite is advantageously combined with other purification and concentration techniques, including ion-exchange chromatography, ligand affinity chromatography, hydrophobic interaction chromatography, ultrafiltration, and differential precipitation.

Method for purifying thrombopoietin

Highly purified factor XIII and methods of purifying factor XIII are disclosed. Factor XIII is purified from a biological fluid, such as a cell lysate. The methods provide factor XIII compositions that are greater than 99% pure with respect to contaminating proteins. The methods further provide factor XIII compositions wherein 1% or less of the factor XIII is activated factor XIII.

Factor XIII compositions

Growing community of inventors

Issaquah, WA, United States of America

Jin-Jyi Chang