Two Cells Co., Ltd.

Hiroshima University

Jin Chang Shao

Yukio Kato

Koichiro Tsuji

Yuki Katsura

Yasufumi Takahashi

Masato Takao

Takashi Matsushita

Shinichi Hasegawa

Minoru Morikawa

Satoshi Maeda

Shinya Miki

Yui Kitayama

Ken Innami

Toshie Tsuchiya

Aki Ohmori

Maiko Hara

Yukio c/o Hiroshima University Kato

A method according to the present invention for producing a cartilage-damage treatment agent, includes the steps of: (i) proliferating mesenchymal stem cells in a serum-free medium A containing an FGF, a PDGF, a TGF-&#x3b2;, an HGF, an EGF, at least one phospholipid and at least one fatty acid; and (ii) mixing the mesenchymal stem cells thus proliferated in the step (i), an isotonic preserving agent, and a cytoprotective agent. This method provides a cartilage-damage treatment agent which favorably regenerates a cartilage tissue.

Cartilage-damage treatment agent and method for producing same

Disclosed are: a culture medium containing a specific growth factor and at least one phospholipid; a composition for preparation of the culture medium; a kit; and a method. A technique can be provided which uses a serum-free or low-serum culture medium and has a promoting effect on the proliferation of an animal cell comparable to the promoting effect obtained by the culture in a serum-containing culture medium.

Culture medium additive for use in serum-free culturing of animal cell, kit and use thereof

Provided is a differentiation-inducing culture medium additive for inducing bone differentiation of at least one type of cell selected from the group consisting of a stem cell, a dental pulp cell, a periodontal ligament cell, a placenta, an amnion, and a fibroblast under a serum-free condition, and a use of the differentiation-inducing culture medium additive. The differentiation-inducing culture medium additive of the present invention for inducing differentiation of a stem cell under a serum-free condition at least contains at least one growth factor selected from the group consisting of EGF, FGF, and PDGF; dexamethasone; and &#x3b2;-glycerophosphate. The differentiation-inducing culture medium additive of the present invention does not require ascorbic acid 2-phosphate and ITS, which are normally essential for bone differentiation. Further, bone differentiation can be promoted by adding phospholipid.

Differentiation-inducing culture medium additive and use thereof

A cell preparation containing mesenchymal stem cells whose immunosuppression ability is maintained is produced by means of a serum-free or low-serum culture. A method for producing a cell preparation containing mesenchymal stem cells, comprising the steps of: (A) proliferating mesenchymal stem cells in a serum-free medium 'A' containing an FGF, a PDGF, a TGF-&#x3b2;, an HGF, an EGF, at least one phospholipid, and at least one fatty acid; and (B) screening mesenchymal stem cells whose immunosuppression ability is maintained or improved, from the mesenchymal stem cells thus proliferated in the step (A).

Cell preparation containing mesenchymal stem cells, and method for producing same

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Hiroshima, Japan

Jin Chang Shao