Samyang Biopharmaceuticals Corporation

Samyang Genex Corporation

Jin-Ah Kim

Ho-Joon Choi

Jai-Young Song

Chang-Heon Kim

The present invention relates to a method of producing secondary metabolites with a high yield using plant cell culture, and a medium for the production of secondary metabolites. More specifically, the method of the present invention is characterized in that the plant cell culture is conducted by adding a saccharide mixture to the culture medium as a carbon source, to increase the productivity of the secondary metabolites. For example, the present invention establishes the method of increasing the productivity of secondary metabolites and shortening the culture time by the use of the mixture of glucose and fructose in the plant cell culture, thereby contributing in producing useful secondary metabolites on an industrial scale using plant cell culture.

Mass production of secondary metabolite in plant cell culture by treatment of saccharide mixture in medium

The present invention relates to a method of producing corosolic acid by using plant cells that produce corosolic acid. More particularly, the present invention relates to a method of producing corosolic acid by using plant cell suspension cultures comprising the steps of: inducing a callus from a tissue of a plant producing corosolic acid; preparing a cell line capable of being cultured in liquid culture medium from the induced callus; culturing the cell line in a suspension culture; and isolating corosolic acid from the culture solution. The present invention has advantage of maximizing productivity by utilizing two-stage culture, treatment with inducing agent, and high cell-density culture in the suspension culture of plant cells producing corosolic acid.

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Jeongeup, South Korea

Jin-Ah Kim