Other

University of California

Jimmy Loon

Paul I Terasaki

Felix H Yiu

Steven Hardiwidjaja

Nadim El-Awar

A composition of matter and method for using the composition of matter to isolate various cell elements from a heterogeneous cell suspension in a simple one step procedure. The composition is a monoclonal antibody mixture including complement and a non-toxic density gradient media. The composition provides a negative selection technique in which undesired subpopulation cells are lysed and separated from the desired cell subpopulation. The invention has particular application to isolation of white cell elements.

Method and composition for isolating white cell elements

A tissue typing tray is provided with an improved well cover. The tissue typing tray is of conventional box-like construction, with conjoining side walls and a bottom and a plurality of wells disposed on the bottom. The well cover comprises a flat member, with a downwardly depending, continuous ridge member inwardly spaced from the perimeter of the flat member and terminating in a flat surface provided with a pressure sensitive adhesive thereon. The ridge member is of the same height as the wells and encompasses the wells. Upon engagement of the well cover with the tissue typing tray, the adhesive-coated ridge member contacts the bottom of the tray, while the flat member of the well cover contacts the tops of the wells. The sealing configuration of the well cover prevents its lateral or vertical movement, thereby preventing possible loss of testing reagent from the wells or contamination of the contents of one well by the contents of another. An optional top cover comprises a flat cover member and a downwardly depending, continuous side wall member around the perimeter thereof for releasable friction-fit engagement with the exterior of the side walls of the tissue typing tray.

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Reseda, CA, United States of America

Jimmy Loon