University of Texas System

The Texas A&m University System

Jimmy Gollihar

Andrew D Ellington

Jared Ellefson

Sanchita Bhadra

Bingling Li

Randy Allen Hughes

Yu Sherry Jiang

Yan Du

Raghav Shroff

Elizabeth Gardner

Katy Kao

Michelle Byrom

Arti Pothukuchy

Embodiments of the disclosure concern methods and compositions related to generation and/or use of proofreading reverse transcriptases, including those that are thermophilic or hyperthermophilic. The disclosure encompasses specific recombinant polymerases and their use. In some embodiments, the polymerases are utilized for RNA sequencing in the absence of generation of a cDNA intermediate.

Thermostable reverse transcriptase

Disclosed are methods for isothermal nucleic acid amplification and detection.

Strand displacement with loop-mediated isothermal amplification

Disclosed herein is a method of utilizing an enzyme in a nucleic acid manipulation process, the method comprising: a) transforming a microorganism with a non-native enzyme; b) inducing expression of the enzyme in the microorganism, thereby producing the non-native enzyme; c) adding the microorganism of step b) directly to a non-naturally occurring nucleic acid manipulation process, wherein the non-native enzyme is not purified from the microorganism prior to addition to the nucleic acid manipulation process; and carrying out the nucleic acid manipulation process using the enzyme. Importantly, this method can be carried out without the need to purify the enzyme from the cell producing it before it is used in the nucleic acid manipulation method. Also disclosed herein is a kit for carrying out a nucleic acid manipulation process, the kit comprising a) a microorganism expressing a non-native enzyme; b) nucleic acids of interest; and c) reagents for use in the nucleic acid manipulation process.

Methods and kits for using recombinant microorganisms as direct reagents in biological applications

Disclosed herein are methods and platforms using Receptor Compartmentalized Partnered Replication (CPR), in which the partner gene is a receptor, signal transduction pathway, or metabolic pathway that leads to the production of an effector molecule for the receptor or signal transduction pathway. The signal transduction pathway or receptor is coupled to the production of a thermostable polymerase. Emulsification of libraries of organisms with primers that can amplify the partner gene led to the selective amplification of those partner genes that were best able to produce the thermostable polymerase during thermal cycling of the emulsion.

Methods and platform for screening and selecting metabolites and their receptors

Disclosed are compositions and methods for isothermal nucleic acid amplification and detection.

Methods and devices related to toehold-based strand displacement with loop-mediated isothermal amplification

Growing community of inventors

Austin, TX, United States of America

Jimmy Gollihar