10x Genomics, Inc.

Jill Herschleb

Adam Lowe

Meiliana Tjandra

Shalini Gohil

Joshua Delaney

Albert Dale Kim

Bill Kengli Lin

Cedric Uytingco

The present disclosure provides compositions and methods for using fixed biological samples in partition-based assays. In at least one embodiment, the disclosure provides a composition comprising a fixed biological sample and an un-fixing agent contained in a partition, such as a discrete droplet. In some embodiments, the disclosure provides un-fixing agent compounds capable of catalytically cleaving crosslinks in fixed biological samples, particularly crosslinked nucleic acids, such as RNA.

Methods for generating barcoded nucleic acid molecules using fixed cells

Compositions and methods for using fixed biological samples

Provided are methods of capturing an analyte from a biological sample using a hydrogel that includes capture probes to capture the analyte, identifying a region of interest of the biological sample, and isolating the region of interest from the biological sample by removing a portion of the hydrogel that corresponds to the region of interest. Compositions and kits for performing the methods are also provided.

Spatial analysis utilizing degradable hydrogels

Growing community of inventors

San Francisco, CA, United States of America

Jill Herschleb