Jiangnan University

Bright Dairy & Food Co., Ltd.

Jieying Deng

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Yang Gu

Xueqin Lv

Chunmei Chen

Yang Song

Yawen Zhao

Ke Liu

The disclosure discloses recombinantfor synthesizing guanosine diphosphate fucose and a construction method and application thereof. The recombinantis obtained by intensively expressing guanylate kinase and nucleotide diphosphokinase genes and expressing exogenous fucokinase and phosphate guanylyltransferase genes in a genome of168. According to the disclosure, a bacterial strain for synthesizing the guanosine diphosphate fucose is obtained by reconstructing the168, with a volume of intracellular accumulation up to 196.15 g/L. According to the disclosure, by intensively expressing the guanylate kinase and nucleotide diphosphokinase genes, and enhancing the supply of intracellular GDP-L-fucose composition cofactors, the synthesis of the guanosine diphosphate fucose is promoted. The construction method for the recombinantof the disclosure is simple and convenient to use, thus having good application prospects.

Recombinantfor synthesizing GDP-L-fucose and application thereof

The present invention provides a recombinantwith improved 2'-fucosyllactose production, and a construction method thereof. In the present invention, a strain capable of efficiently synthesizing 2&#x2032;-fucosyllactose is obtained by the fusion expression of the fucosyltransferase gene and the L-fucokinase/guanosine 5&#x2032;-diphosphate-L-fucose pyrophosphorylase gene inBSGL-FF, the fermentation supernatant of which comprises a cumulative amount of 2&#x2032;-fucosyllactose as high as 1.62 g/L, which is 55% higher than the amount achieved with the control strain. The construction method of the recombinantof the present invention is simple, and convenient to use, and thus has good application prospects.

Recombinantwith improved 2'-fucosyllactose production, and construction method thereof

The present invention relates to a method for promoting acetylglucosamine synthesis of, which belongs to the field of genetic engineering. The present invention adopts the recombinantBSGNKAP2 as a starting strain, exogenously introducing pyruvate carboxylase BalpycA derived from, eliminating the central carbon metabolism overflow of theand avoiding the synthesis of the by-product acetoin; further, five exogenous reducing force metabolic reactions are introduced to replace the reaction of generating NADH in glycolysis pathway and tricarboxylic acid cycle to reconstruct intracellular reducing force metabolism, which specifically comprise glyceraldehyde-3-phosphate ferredoxin dehydrogenase, isocitrate NADdehydrogenase, a malate quinone dehydrogenase, a ketoacid ferredoxin oxidoreductase and a nitrogenase ferritin. In a shake-flask fermentation process using a complex medium, acetylglucosamine yield of the recombinant strain BSGNKAP8 is 24.50 g/L, acetylglucosamine/glucose yield is 0.469 g/g, respectively 1.97 times and 2.13 times of those of the starting strain BSGNKAP2.

Method for promoting acetylglucosamine synthesis of bacillus subtilis

The invention relates to a recombinant strain of, wherein pyruvate carboxylase BalpycA, glyceraldehyde-3-phosphate ferredoxin dehydrogenase gor, isocitrate NADdehydrogenase icd, malate quinone dehydrogenase mqo, pyruvate ferredoxin oxidoreductase porAB and nitrogenase ferritin cyh are integrated and expressed in the recombinant strain. The invention also discloses use of the recombinant strain in fermentation production of acetylglucosamine. The recombinantof the invention eliminates the central carbon metabolism overflow of theand balances the intracellular reducing force, and the fermentation yield of acetylglucosamine is greatly improved.

Recombinant strain of

The invention provides an effective method for improving N-acetylglucosamine (GlcNAc) production by engineeredDeletion of phosphoenolpyruvate carboxykinase encoding gene pckA and encoding pyruvate kinase gene pyK in recombinant GlcNAc-producing strain BSGNK-PxylA-glmS-P43-GNA1 (BSGNK) is first performed to enhance GlcNAc production, followed by overexpression of pyruvate carboxylase encoding gene pycA for facilitating cell growth. Finally, the GlcNAc production of the recombinant strain BPTS3 reached to 11.3 g/L, which was 1.84-fold of BSGNK. This method can be used for improve cellular property of engineeredfor GlcNAc production, which can be further applied to industrial production of GlcNAc.

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Wuxi, China

Jieying Deng