The Furukawa Electric Co., Ltd.

Yamato Scientific Co., Ltd.

Jie Xu

Ken Tsukii

Toru Takahashi

Kenichi Kimura

Kenji Suzuki

Mariko Matsunaga

Shinichi Taguchi

Takayuki Matsumoto

Masato Shiino

Satoshi Sugiyama

Shinichiro Iizuka

Isamu Kinoshita

Etsuo Tanabe

Kohta Igarashi

Motosuke Kiyohara

A cell accommodating chip is adapted for use in a screening apparatus for searching for a predetermined cell based on optical information emitted from a substance on the cell accommodating chip and selectively collecting the cell searched for. The cell accommodating chip includes a substrate composed of a light-transmitting material, and a plurality of wells capable of accommodating cells, the plurality of wells being provided on at least one of main faces of the substrate. A surface of the cell accommodating chip having the plurality of wells is coated with a specific polymer having a crosslinked structure.

Cell accommodating chip

A cell accommodating chip capable of accommodating a plurality of cells includes a substrate composed of a light-transmitting material, and a plurality of wells on at least one of main faces of the substrate, the plurality of wells being capable of accommodating cells. A surface of the cell accommodating chip including the plurality of wells has a low cell adhesion property, and affinity to a specific binding material having affinity to a produced substance produced by a cell accommodated in one of the wells and/or a released substance released by the cell.

Cell accommodating chip and screening method using the cell accommodating chip

A screening apparatus for searching for a predetermined microparticle based on optical information emitted from microparticles to selectively pick up the microparticle searched for includes a measurement chip that is made of a light permeable material, the measurement chip having a well formed therein that retains a liquid including at least one microparticle, a measuring section that is configured to acquire optical information emitted by the microparticles retained in the measurement chip, an analyzing section that is configured to analyze the optical information to extract optical information associated with the microparticles retained in the well, a liquid retaining section provided on the measurement chip, a draining section that is configured to drain a liquid retained in the liquid retaining section, an introducing section that introduces a liquid into the liquid retained section, and a liquid level controlling section that controls a liquid level of the liquid retaining section.

Screening apparatus and screening method

A screening apparatus includes a light permeable measurement chip having a well retaining a liquid including microparticles, a measuring section acquiring optical information of the microparticles obtained by illuminating the microparticles, an analyzing section that extracts optical information of the microparticles, a receiving plate receiving a microparticle selectively picked up from the measurement chip, a moving section moving the measurement chip and the receiving plate against the measuring section, and a collecting section for collecting a microparticle by sucking with the suction-ejection capillary and ejecting on the receiving plate. A distal-end outer dimension of the capillary is greater than a width of the well. The capillary sucks the target microparticle at a position where the distal end of the capillary and the measurement chip are spaced by a predetermined distance and a central axis of the distal end of the capillary and a central axis of the well are mutually displaced.

The invention provides an optical measuring apparatus having a plurality of measuring sections each having a light irradiating section for irradiating light to specimens and a light receiving section for receiving optical data acquired by irradiating the light to the specimens and a flow rate calculating section for calculating flow rate values of the specimens based on a difference of measured times of the optical data measured by the plurality of measuring sections with respect to the specimens and a distance between the plurality of measuring sections. The optical measuring apparatus measures the optical data of the specimens by irradiating light to the specimens, i.e., the objects to be measured, dispersed within a sample fluid flowing through a flow passage. The optical measuring apparatus further includes a flow rate graph generating section for generating flow rate graph data in which the values of flow rate of the specimens calculated by the flow rate calculating section are arrayed in order of calculation in time-series manner and for outputting the generated flow rate graph data to a displaying section.

Optical measuring apparatus and specimen discriminating and dispensing apparatus

A sample identification/sorting apparatus includes a sample storage portion that stores samples dispersed in a liquid, a flow cell having a flow path through which the liquid flows, and a sorting nozzle that has a flow path communicating with the flow path of the flow cell and dispenses a sorting solution including the sample, which is a target sample, to a culture plate. A difference between a flow direction of the liquid at an outlet of the sample storage portion and a flow direction of the liquid at a leading end of the sorting nozzle is less than 90 degrees.

Sample identification/sorting apparatus and sample identification/sorting method

A specimen identification and dispensation device includes an optical measurement device which is an identification part for measuring and identifying optical information on a specimen by emitting exciting light to the specimen being a measurement target dispersed in a liquid flowing through inside of a capillary, a dispensation part for dispensing the identified specimen into wells being sections to be dispensed through a nozzle, and a concentration adjustment part which adjusts the number of the specimens contained in an aliquot solution to a desired number according to the concentration of a sample liquid and the amount of the aliquot solution. The dispensation part is movable three-dimensionally with respect to the identification part and the nozzle.

Specimen identification and dispensation device and specimen identification and dispensation method

A sample identification sorting apparatus includes an identifying unit having an optical information-measuring section that measures optical information of a sample dispersed in a liquid, and a determining section that determines whether the sample is a target sample or a non-target sample, a sorting unit including a sorting nozzle having a flow path in communication with the flow path of the identifying unit, a liquid waste-collecting section that collects by suction a liquid waste discharged from a sorting nozzle tip, and a container to collect a sorting solution containing a target sample, a moving unit and a control unit that cause the sorting nozzle and/or the collecting container to move relatively based on the optical information. The liquid waste-collecting section has a suction nozzle that sucks a liquid waste containing a non-target sample discharged from the sorting nozzle tip or containing a non-target sample or a non-sortable sample.

Sample identification sorting apparatus and sample identification sorting method

A method for distinguishing and sorting cells characterized by comprising distinguishing and sorting a specific cell mass or a part of the cells in the cell mass with the use of transmitted light data reflecting the morphological characteristics of the cells such as size and shape optionally together with side-scattering light data reflecting the characteristics of the internal structure of the cells. The part of the cells in the specific cell mass as described above are at the G1 stage or at a part of the M stage in the cell cycle. A part of the cells at the G1 stage are referred to as the left bottom line in an analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data, while a part of the cells at the M stage are referred to as the right bottom line in the analytical dispersion diagram of the cells wherein the abscissa indicates the transmitted light data.

Method for distinguishing and sorting of cells and device therefor

A photodetector detects fluorescence emitted from a sample placed on a substrate of a DNA chip. There is an irradiation optical system for guiding irradiation light by a first optical waveguide, gathering the irradiation light by a first lens and irradiating the sample. A reception optical system gathers fluorescence at an input-side end surface of a second optical waveguide by a second lens and guides the fluorescence to a measuring unit. The irradiation optical system and the reception optical system are separate light guiding paths. The reception optical system is of a confocal optical system in which a focal point on the sample is identical to a focal point at the input-side end surface of the second optical waveguide of the reception optical system.

Photodetector and measurement object reader

A specimen sorter includes a separating device to remove a specimen from a container, a detection device to identify the specimen as a target specimen or a non-target specimen, and a dispensing device to dispense the specimen. The dispensing device includes a dispensing nozzle to contact a vibration member to form liquid droplets at a front end of the dispensing nozzle and to dispense the liquid droplets, a first container to collect the liquid droplets dispensed from the dispensing nozzle that contain the target specimen, and a second container to collect the liquid droplets dispensed from the dispensing nozzle that contain the non-target specimen.

Methods of separating, identifying and dispensing specimen and device therefor, and analyzing device method

An optical information analyzer () includes a light emitting unit () that emits light (excitation light) (L) to a sample (S), a transmission light receiving unit () that receives transmission light (L), which is the excitation light passing through the sample (S), and detects the received transmission light as a transmission light signal (SG), scattered light/fluorescence receiving units () and () that are provided at a plurality of positions, receive side scattered light/fluorescence components (L) and (L) from the sample (S), and detect the received side scattered light/fluorescence components as scattered light/fluorescence signals (SG) and (SG), and an analyzing unit () that measures the optical information of the sample (S) on the basis of the detected scattered light/fluorescence signals (SG) and (SG) and the detected transmission light signal (SG) and analyzes the sample (S) on the basis of the measured optical information.

Optical information analyzer and optical information analysis method

An optical information analyzing device includes an irradiating unit that irradiates irradiation light to specimens, a transmitted light receiving unit that receives transmitted light and detects the transmitted light as a transmitted light signal, a scattering/fluorescent light receiving unit that receives lateral scattering light and fluorescent light and detects the lateral scattering light and the fluorescent light as a scattering/fluorescent light signal, a nozzle position adjusting mechanism, and an analyzing unit that measures the optical information on the specimen on the basis of the detected transmitted light signal and the detected scattering/fluorescent light signal and analyzes the specimen.

Optical information analyzing device and optical information analyzing method

An optical measurement apparatus can be provided, in which the sample is optically measured without loss of the illuminating light with high sensitivity. A glass plate as the transparent memberis placed in the interface between the end faceof the optical waveguideguiding the illuminating light L generated by the laser light sourceand the wall face of the capillary. According to the above feature, the air layer is prevented from existing in the interface between the end faceof the optical fiberand the wall face of the capillary, thus the sample S can be optically measured with high sensitivity and few variability without causing the loss of the illuminating light L.

Method for optical measurement and optical measurement apparatus

[PROBLEMS] A separation unit can confirms a separation state, a detection unit enhances a lighting efficiency and a light reception sensitivity, and a dispensing unit ensures the normal state of a specimen. [MEANS FOR SOLVING PROBLEMS] A specimen separation device characterized by comprising a container for storing a specimen, a nozzle for sucking and ejecting the specimen from the container, a nozzle operating means for moving the nozzle vertically and laterally, and a nozzle controlling means for controlling the suction force and ejection force of the nozzle. A specimen identification device comprising a nozzle for sucking a specimen from a container storing the specimen, a flow path for allowing the specimen to flow therethrough, and a measuring unit having a monitoring light lighting unit and a light receiving unit for observing (monitoring) the specimen that are installed in a flow path, characterized in that the above nozzle, the flow path and the measuring unit are formed in an integrated structure.

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Tokyo, Japan

Jie Xu