Jiangsu Academy of Agricultural Sciences

Jibo Hou

Liting Hou

Xiaoming Yu

Yuanpeng Zhang

Qisheng Zheng

Jin Chen

Xuwen Qiao

Yu Lu

Xiaoyan Zhang

Pengcheng Li

Fang Lv

Yanhong Zhao

Bihua Deng

Jinqiu Zhang

Xiaoxin Zuo

Yiwei Wang

The present invention relates to the preparation of the compound immunopotentiator and the application thereof in a foot-and-mouth disease vaccine of pigs. According to the present invention, the foot-and-mouth disease vaccine of pigs is taken as a research subject, and on this basis, several immunopotentiators having obvious immunopotentiating effects are selected for the compound immunopotentiator, and an antigen/vaccine is mixed with the immunopotentiator to prepare a vaccine-immunized pig. An animal experiment result shows that the present invention has obvious immunopotentiating effects. After immunizing the vaccine containing the compound immunopotentiator, a window period for antibody production can be significantly shortened to 7 days; a LPB-ELISA antibody titer is significantly improved, and an antibody pass rate is significantly increased; an immune protection period is also significantly extended, at least up to 7 months.

Immunopotentiator, foot-and-mouth disease inactivated vaccine and preparation method thereof

A method for inactivated foot-and-mouth disease virus antigen purification and concentration, which relates to the field of biotechnology which includes the following steps of: (1) adding an adaptor protein with an amino acid sequence as shown in SEQ ID NO: 2 into the inactivated foot-and-mouth disease virus antigen, mixing evenly, and incubating; (2) adding a purified carrier, mixing evenly, and incubating the mixture, the purified carrier being askeleton; and (3) centrifuging and obtaining a precipitate.

Method for purifying and concentrating inactivated antigen of foot-and-mouth disease virus

A thermostable formula comprises the following components in percentage by mass: 1% to 5% raffinose, 5% to 10% maltose, 15% to 30% saccharose, 1% to 5% lactose, 1% to 5% glucose, 0.1% to 1.5% polysorbate 80, 0.1% to 0.5% polyethylene glycol 8000, 0.5% to 3% tyrosine, 3% to 6% silk fibroin, and the balance of water for injection. It further discloses a room-temperature-preserved live classical swine fever vaccine and a preparation method thereof, wherein the live classical swine fever vaccine is obtained by mixing the thermostable formula with a live classical swine fever virus solution and then carrying out gradient vacuum drying. The vaccine prepared according to the present invention has a dried foam appearance and presents a glass-layer like structure under a scanning electron microscope, has a glass transition temperature up to more than 50&#xb0; C.

Heat-resistant protective agent, room-temperature-preserved live classical swine fever vaccine, and preparation method and application thereof

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Nanjing, China

Jibo Hou