Hoboomlife Bio-technology (shenzhen) Co., Ltd.

Shenzhen Sosen Electronics Co., Ltd

Favoru Electronic Co., Ltd.

Jiansheng Chen

Haichao Zhang

Liqing Zhao

Zhigang Duan

Beijia Huang

Ping Wang

Bo Zhang

Jun Fang

Chaoyang Zou

Ping Dai

Tong Zhang

Yaying Hou

The present disclosure provides an innovative method for improving the enzyme activity of an NMN biosynthetic enzyme Nampt, and relates to the technical field of genetic engineering. A mutant protein of the present disclosure is obtained by firstly analyzing a target protein Nampt using two softwares FoldX and DeepDDG, and then predicting multiple key sites influencing the enzyme functions and finally performing the semi-rational design of the enzyme. In the examples of the present disclosure, 10 mutant strains are constructed using the designed primers according to the principle of point mutation, and 8 of the mutants have higher activity than a wild-type strain, in which the NMN yield of the mutant Nampt-V365L is increased by 62%, and the NMN yields of the mutants Nampt-S248A, Nampt-N164L, Nampt-S382M, Nampt-A245T and Nampt-A208G are increased by 34%, 27%, 27%, 22% and 17% respectively.

Innovative method for improving enzyme activity of NMN biosynthetic enzyme nampt

The present invention discloses a microcapsule powder stable in gastric acid, a method for preparing the same and use thereof. The microcapsule powder comprises a core material and a capsule material coated outside the core material, wherein the capsule material has a melting point of greater than 42&#xb0; C., and the capsule material does not decompose or dissolve under the action of protease and gastric acid, but decomposes under the action of intestinal digestive enzymes. The core material is coated with the capsule material in the microcapsule powder, thus achieving high-efficiency coating of the core material by a single component. The microcapsule powder achieves conventional intragastric stability as well as favorable stability in an open environment at room temperature, thus solving the problem that the conventional embedding solution may only achieve intragastric stability, but the stability in an open environment at room temperature is low.

Microcapsule powder stable in gastric acid, method for preparing same, and use thereof

Innovative method for improving enzyme activity of NMN biosynthetic enzyme Nampt

The application relates to an over-temperature protection circuit of an LED driving power supply. The over-temperature protection circuit comprises a temperature detection circuit for sensing a temperature change; a switch control circuit connected to the output end of the temperature detection circuit and controlling the on-off of the circuit according to the output signal of the temperature detection circuit; a shunt circuit connected to an output end of the switch control circuit and shunting current when the switch control circuit is in an on state; a current detection circuit connected to an output end of the shunt circuit and changing a current detection signal according to an output current of the shunt circuit; the output end of the current detection circuit is connected with the feedback loop. The application has the advantages of low cost, stable performance, little environmental influence and high precision.

Over-temperature protection circuit of an LED driving power supply

Neck pillow

The present invention relates to a plug-in bulb coupling structure, comprising a lamp cap provided on a bulb and a plug-in lamp holder provided on a power line, wherein a plug-in end of the plug-in lamp holder is provided with plug-in holes and raised barbs, and conductive tubes connected to a lead are provided in the plug-in holes; the plug-in end of the plug-in lamp cap is provided with conductive bars and barb holes, and the conductive bars are connected to a drive circuit board in the plug-in lamp cap. With the coupling structure of the present invention, the drive circuit board and the SR of the power line can be assembled and connected to each other quickly. The operation is easy, convenient and quick and does not require any tools.

Plug-in bulb coupling structure

Growing community of inventors

Guangdong, China

Jiansheng Chen