Dow Agrosciences LLC

Corteva Agriscience LLC

Jianquan Li

Steven Lee Evans

Joel Jay Sheets

Joseph F Petolino

Ryan C Blue

Kenneth Edwin Narva

Timothy Denver Hey

Thomas Meade

Todd P Glancy

Krishna M Madduri

James Edward King

Sek Yee Tan

Gaofeng Lin

Ryan M Lee

Janna Mai Armstrong

Audrey Jane Etter

James M Hasler

Tristan E Coram

James E King

Steven L Evans

This disclosure concerns compositions and methods for increasing the expression of a polynucleotide of interest. Some embodiments concern novel transactivation polypeptides and variants thereof that have been identified in plants, and methods of using the same. Particular embodiments concern the use of at least one DNA-binding polypeptide in a fusion protein to target at least one transactivation polypeptide or variant thereof to a specific binding site on a nucleic acid comprising the polynucleotide of interest, such that its expression may be increased.

Plant transactivation interaction motifs and uses thereof

The subject invention concernsmodified Cry1Ca insecticidal toxins and the polynucleotide sequences which encode these toxins. Uses in transgenic plants are described as are methods for protecting crops from insect pest damage.

Modified Cry1Ca toxins useful for control of insect pests

The invention is drawn to a method for purifying membrane-bound proteins expressed in recombinant insect cells using N-laurosarcosine. The invention is particularly suited for expressing cadherin-type receptors cloned from, European Corn Borer, and expressed in Sf9 insect cells. The method is optionally adapted for use with 6-his tag proteins.

Methods for purifying insect membrane-bound receptor proteins from recombinant production hosts

Cysteine-specific radiolabeled Cry1Fa protein retains insecticidal activity against insect pests and binds to insect brush border membrane vesicle receptors in a saturable manner. The biologically-active radiolabeled Cry1Fa protein is useful in competitive binding assays with other Cry toxins.

Biologically-active radiolabeled Cry1Fa and receptor binding assay methods

Growing community of inventors

Zionsville, IN, United States of America

Jianquan Li