University of Michigan

Jianping Fu

Kevin R Ward

Zida Li

Sajedeh Nasr Esfahani

Kenichiro Taniguchi

Yi Zheng

Deborah Gumucio

Xiang Li

Yue Shao

The present disclosure provides compositions and methods employing stem cell-derived amnion tissue. In some embodiments, compositions (e.g., scaffolds and devices) and methods of generating amnion-like tissues from hPSCs are provided. In some embodiments, uses of such cells for research, compound screening and analysis, and therapeutics are provided.

Development of amnion-like tissue from human pluripotent stem cells

A blood clot retraction assay device and methods of measuring whole-blood clot retractive forces during coagulation are described. When placed in proximity to a micro-beam, a blood droplet attaches thereto, applying a force to the micro-beam during clot formation. Using Piezoresistive micro-beams or other Piezoresistive components disposed upon such micro-beams, the device measures retractive force of a blood droplet based upon changes in resistance. The sensitivity of such measurements allows use of small quantities of blood for measurement, between 15 microliters and 300 microliters. The device further permits continuous measurement of coagulation, allowing measurement of distinct reaction, contraction development, and fibrinolysis phases for each blood sample.

Microscale whole blood coagulation assay platform

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Ann Arbor, MI, United States of America

Jianping Fu