E.i. Dupont De Nemours and Company

Jianjun Yang

Narendra S Yadav

Perry G Caimi

Luan Tao

Paul Veikko Viitanen

Min Qi

Perry G G Caimi

Robel Y Kahsay

Synthetic, derivative promoters for expression of chimeric genes in, and related bacteria were created. The promoters have a C to T change in the nucleotide at position 90 of theglyceraldehyde-3-phosphate dehydrogenase gene promoter. Promoters with this change have higher expression as compared to the native promoter from which they are derived and are useful for genetic engineering for expressing coding regions or regulatory RNA to obtain high levels of expressed proteins or regulatory RNAs.

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The endogenous pnp gene encoding polynucleotide phosphorylase in thegenome was identified as a target for modification to provide improved xylose utilizing cells for ethanol production. The cells are in addition genetically modified to have increased expression of ribose-5-phosphate isomerase (RPI) activity, as compared to cells without this genetic modification, and are not limited in xylose isomerase activity in the absence of the pnp modification.

expressing xylose isomerase fromwas found to have improved xylose utilization, growth, and ethanol production when grown in media containing xylose. Xylose isomerases related to that ofwere identified structurally through molecular phylogenetic and Profile Hidden Markov Model analyses, providing xylose isomerases that may be used to improve xylose utilization.

The present invention provides methods for the conditional or regulated expression of a site-specific recombinase using split intein-mediated protein splicing. This enhances the temporal and tissue-specificity of trait gene expression and allows for fine tuning of expression specificity.

Method of controlling site-specific recombination

The invention provides methods for the production of silks and silk-like proteins (SLP's) in green plants. Expression of SLP's has been achieved in both seed and leaf tissue in green plants.

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Hockessin, DE, United States of America

Jianjun Yang