Anaspec, Inc.

Aat Bioquest, Inc.

Jianjun He

Zhenjun Diwu

Vera Rakhmanova

Ming Zhu

Anita Hong

Raman Khosrow Afshar

Yi Tang

Jinfang Liao

Qingzheng Wang

Qingheng Wang

Fluorescent dyes useful for preparing fluorescent metal ion indicators, the fluorescent indicators themselves, and the use of the fluorescent indicators for the detection, discrimination and quantification of metal cations.

Fluorescent ion indicators and their applications

The present invention is generally directed to thiol quantitation assays, methods of performing the assays, and compounds used in the assays. It is more specifically directed to assays that include one or more disulfides and related molecules and methods. The disulfides contain a FRET pair.

Compounds related to assays

The present invention relates generally to the detection and measurement of transmembrane potentials using an N,N,N'-trialkyl thiobarbituric acid-derived polymethine oxonol (shown below). In particular, the present invention is directed to compositions and optical methods for determining transmembrane potentials across the plasma membrane of biological cells using a slightly hydrophobic N,N,N&#x2032;-trialkyl thiobarbituric acid-derived polymethine oxonols. The method comprises a slightly hydrophobic N,N,N&#x2032;-trialkyl thiobarbituric acid-derived polymethine oxonol anion capable of redistributing from a first face of the membrane to a second face of the membrane in response to changes in the potential of the membrane. In one aspect the method is used to identify compounds which modulate membrane potentials in biological membranes. wherein R1, R2, and R3 are independently selected from the group consisting of hydrogen, alkyl, haloalkyl and heteroalkyl; n is an integer from 1 to 3; Z is Na, K, ammonium or other biologically acceptable salt.

Detection of transmembrane potentials using N,N,N'-trialkyl thiobarbituric acid-derived polymethine oxonols

Thiol quantitation assays and related methods

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Sunnyvale, CA, United States of America

Jianjun He