Shenzhen Mindray Bio-medical Electronics Co., Ltd.

Jianhui Shao

Bing Xu

Ting Lei

Li Zhang

Xiaojun Peng

Jiangli Fan

Shiguo Sun

Tong Wu

Yumei Zhao

Yuji Kuang

Yang Zhao

Bingshuai Wang

The present disclosure provides a reagent for blood analysis which may include: (1) a compound having the general formula I as a fluorescent dye, wherein n, X, R, R, R, R, Rand Yare as defined in the specification; (2) a surfactant selected from cationic surfactants, zwitterionic surfactants and anionic surfactants. The present disclosure also provides a method to perform blood analysis including the following steps of: (a) mixing the blood sample with the reagent for blood analysis disclosed to form a cell suspension; (b) detecting the scattered light signals and fluorescence signals from the cells; and (c) differentiating and counting the cells in the blood in terms of the scattered light signals and fluorescence signals. 

Reagent for blood analysis and method of use thereof

Cyanine compounds having the general formula I for staining biological samples, wherein R, R, X, Y, Aand Aare as defined in the specification. These kinds of compounds may show good light illumination stability, have a maximum absorption peak around 640 nm that may not change as a function of ambient temperature, have rapidly increased fluorescence intensity upon binding to nucleic acids to form compound/nucleic acid complexes, and have a light spectrum in the near-infrared region, thereby effectively reducing interference from background fluorescence and increasing the accuracy of the detection when used as a staining agent for nucleic acids in a flow cytometer. The compounds provided can be used as a staining agent for erythroblasts in the blood. 

Cyanine compounds and their use in staining biological samples

A compound having the general formula I or a conjugate thereof, wherein various groups are as defined in the specification. A composition includes: (i) a compound having the general formula I or a conjugate thereof; and (ii) at least one surfactant selected from cationic surfactants and nonionic surfactants. Also disclosed is a preparation method for the composition and a kit comprising the composition. Further disclosed is a method for identifying and differentiating erythroblasts, basophils and lymphocytes simultaneously using the composition according to the present disclosure. 

Cyanine compounds, compositions including these compounds and their use in cell analysis

Asymmetric cyanine fluorescent dyes are represented by general formula I. These kinds of dyes may be used as a staining agent for nucleic acids, with the spectra at 600-900 nm in the near-infrared region and without interference from background fluorescence. These kinds of dyes may be useful with small-type red semiconductor lasers as the light source (such as 633 nm). Compositions comprising these dyes and methods for staining biological samples using these dyes or compositions are also provided. 

Asymmetric cyanine fluorescent dyes, compositions and their use in staining biological samples

The present disclosure provides the asymmetric cyanine fluorescent dyes of formula I in which X, n, R, R, R, R, Rand Yare as defined in the specification. The present disclosure also provides conjugates of the fluorescent dyes, methods for preparation thereof, compositions comprising the fluorescent dyes, and methods for staining biological samples using the fluorescent dyes and compositions thereof. 

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Shenzhen, China

Jianhui Shao