Jiangnan University

Shandong Runde Biotechnology Co., Ltd

Jiangong Lu

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Xueqin Lv

Changfeng Liu

Jianxing Lu

Chen Deng

The present invention discloses a RamA transcription factor mutant for promoting the production of N-acetylglucosamine and use thereof. The mutant is obtained by mutating lysine at position 90 to asparagine and serine at position 92 to lysine in a parent having an amino acid sequence as shown in SEQ ID NO: 2. The present invention provides a genetically engineered strain that overexpresses the RamA transcription factor mutant and increases the production of N-acetylglucosamine. By overexpressing the transcription factor RamA that is involved in the regulation of carbon metabolism, the extracellular accumulation of N-acetylglucosamine is increased, with a maximum concentration reaching 31.5 g/L, which lays a foundation for further metabolic engineering ofto produce glucosamine. The method for constructing recombinantof the invention is simple, and convenient in use, and thus has good application prospects.

RamA transcription factor mutant for promoting production of N-acetylglucosamine and use thereof

The present invention provides a recombinantproducing N-acetylglucosamine and use thereof. The recombinantis obtained by overexpressing, in, the transcription factor SugR derived therefrom. The recombinantof the present invention increases the production of acetylglucosamine to up to 26 g/L, and lays a foundation for further metabolic engineering ofto produce glucosamine.

Transcription factor SugR coding gene, and use thereof in production of N-acetylglucosamine

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Taian, China

Jiangong Lu