Nantong Licheng Biological Engineering Co. Ltd

Jianghua Li

Qinqing Gu

Jiangbo Li

Hongzhi Xia

Shixiu Cui

Long Liu

Jian Chen

Xueqin Lv

Guocheng Du

The present disclosure provides a recombinantfor increasing the yield of menaquinone 7 (MK-7) and application thereof, and belongs to the field of genetic engineering. In the present disclosure, 14 recombinant strains BS1-BS14 are constructed through the modification of genes related to the biosynthetic pathway of MK-7 on a chromosome of, wherein BS6-BS14 significantly increase the yield of the MK-7, reaching up to 33.5 mg/L, which is 3.53 times the yield of the original strain of wild-type168. The present disclosure further provides a method for modifying the MK-7 biosynthetic pathway in microorganisms to increase the yield of the MK-7, providing a theoretical basis for constructing a high-yielding strain of the MK-7.

Recombinantfor increasing yield of menaquinone 7 and application thereof

Growing community of inventors

Jiangsu, China

Jianghua Li