Shaanxi University of Science and Technology

Jian Zha

Bin Tian

Qianqian Zhao

Gennian Mao

Yongbo Wang

Chunyang Shi

Han Li

Dan Yang

Jingyi Li

Liang Xin

Liang Qi

Wenbo Yao

Jingwen Xu

Chengyuan Liang

Xingke Ju

Yuzhi Liu

Yonghong Tang

Ruina Bian

Huayin Pu

Zhiqiang Liu

Yanghan Liu

Guoli Gong

Runcong Sun

A method for extracting protein without lysing cells includes: step 1: combining a penetrating peptide CPP gene having a sequence as set forth in SEQ ID NO: 1 and a bacterial lyase T4L gene having a sequence as set forth in SEQ ID NO: 2 through a flexible connecting peptide GGGGS gene having a sequence as set forth in SEQ ID NO: 3 to form a fusion enzyme CPP-T4L gene having a sequence as set forth in SEQ ID NO: 4; step 2: inserting the fusion enzyme CPP-T4L gene, and obtaining a recombinant host strain; step 3: cloning a target protein expression gene, and then constructing a recombinant expression strain; step 4: first inducing the expression of the target protein expression gene; starting the expression of the fusion enzyme CPP-T4L gene, and releasing a target protein; step 5: collecting cell lysis supernatant to recover the target protein.

Protein extraction method without lysing cells

A compound with anti-drug resistant bacteria activity having the following formula (I): (I) is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

Pleuromutilin salicylic acid ester with antibacterial activity and a method of preparing the same

A compound with anti-drug resistant bacteria activity having the following formula (I) is disclosed. The methods of preparing the compound of formula (I) are also disclosed.

Pleuromutilin hippuric acid ester with antibacterial activity and a method of preparing the same

A compound having the formula (I): is disclosed. A method of preparing the compound of formula (I) is also disclosed.

Hydroxytyrosol isophorone diisocyannate derivative with antioxidant activity and a method of preparing the same

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Xi'an, China

Jian Zha