Cargill Inc.

Michigan Biotechnology Institute

Yi, Jian

Jian Yi

Holly J Jessen

Aristos Aristidou

Pirkko Suominen

Hans H Liao

Ana Negrete-Raymond

Michael V Guettler

Susanne Kleff

Joshua Lundorff

The present application discloses the identification of the novelxylose transporter genes KHT105 and RAG4, as well as the identification of a novel set ofpentose phosphate pathway genes The present application further discloses a series of genetically modified yeast cells comprising various combinations of arabinose fermentation pathways, xylose fermentation pathways, pentose phosphate pathways, and/or xylose transporter genes, and methods of culturing these cells to produce ethanol in fermentation media containing xylose.

Compositions and methods for increased ethanol production from biomass

The present application discloses the identification of novelADH1, ADHa, and ADHb genes, and the production and characterization of genetically modified yeast cells in which these genes were altered. Provided herein are isolatedADH1, ADHa, and ADHb polynucleotides and polypeptides, genetically modified yeast cells that overexpressADH1 and/or contain deletions or disruptions of ADHa and/or ADHb, and methods of using culturing these modified cells to produce ethanol.

Compositions and methods for increased ethanol titer from biomass

Disclosed are recombinant microorganisms for producing organic acids. The recombinant microorganisms express a polypeptide that has the enzymatic activity of an enzyme that is utilized in the pentose phosphate cycle. The recombinant microorganism may include recombinantthat has been transformed to express a Zwischenferment (Zwf) gene. The recombinant microorganisms may be useful in fermentation processes for producing organic acids such as succinic acid and lactic acid. Also disclosed are novel plasmids that are useful for transforming microorganisms to produce recombinant microorganisms that express enzymes such as Zwf.

Recombinant microorganisms for increased production of organic acids

Growing community of inventors

Carlsbad, CA, United States of America

Jian Yi