Illumina Corporation

Illumina Cambridge Limited

Jian-Sen Li

Grace DeSantis

Andrew Slatter

Stephen M Gross

Samantha Snow

Natalie Morrell

Kevin Shen

Ali Asadi

Junhua Zhao

Oliver Jon Miller

Hayley Marianne Hogan

This disclosure describes a hybridization buffer including a crowding agent, a method that includes using the hybridization buffer, and a kit including the hybridization buffer. This disclosure also describes blockers for use in hybrid capture methods, methods of using those blockers, and a kit including those blockers. Additionally, this disclosure describes a method of hybrid capture that does not include amplifying the library members using PCR prior to sequencing the library members.

Compositions and methods for improving library enrichment

The present disclosure relates to methods, compositions, and kits for treating target nucleic acids, including methods and compositions for fragmenting and tagging nucleic acid (e.g., DNA) using transposome complexes bound to a solid support.

Tagmentation using immobilized transposomes with linkers

Provided herein are processes for preparing fluorescent 1-cyano-2-substituted isoindole compounds or N-substituted phthalazinium compounds, comprising reacting an aromatic dialdehyde or aromatic aldehyde-ketone compound with a material that contains primary amino or hydrazine groups, and assaying methods involving the processes thereof.

Fluorescent detection of amines and hydrazines and assaying methods thereof

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San Diego, CA, United States of America

Jian-Sen Li