Cepheid Corporation

Ael Mining Services Limited

Ceoheid

Wako Pure Chemical Industries, Inc.

Jian Ping Zhang

David Swenson

Alan Wortman

Robert Ruhfel

Ernest Jay Friedlander

Ronald G Chang

Jesus Ching

Douglas Bryan Dority

Reuel Van Atta

Wendy Wong

Kendra Lara Paul

James Jian Quan Wang

Chen Li

Samuel Chan

James Jian Wang

Wendy Wingkei Wong

Alan Thomas Wortman

The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.

DNA recombination junction detection

A device is presented that includes a chip having a plurality of wells that are optionally connected by capillary channels, and a manifold member configured to be disposed over the chip for equalizing pressure over the wells and capillary channels.

Pressure manifold to equalize pressure in integration PCR-CE microfluidic devices

The invention is directed to systems, methods, and apparatus for carrying out multi-stage amplification reactions, especially under fluidly closed conditions. In one aspect, methods of the invention are carried out in a fluidly closed reaction system that permits the isolation of a portion of a first (or prior) reaction mixture and its use as a sample or specimen in a second (or subsequent) reaction mixture, thereby substantially avoiding interfering effects that first reaction components may have in the second reaction if both reaction mixtures were simply combined together. In this aspect, systems, methods, and apparatus of the invention may be used with any amplification reaction that permits multiple stages of amplification based on the use of nested primers.

Closed-system multi-stage nucleic acid amplification reactions

Growing community of inventors

Moraga, CA, United States of America

Jian Ping Zhang