Nutrition & Biosciences USA 4, Inc.

E.i. Dupont De Nemours and Company

Dupont Industrial Biosciences Usa, LLC

Jian Ping Lai

Qiong Cheng

Kristin Ruebling-Jass

Robert DiCosimo

Zheng You

Arthur Ouwehand

Mark Scott Payne

Yougen Li

Steven Cary Rothman

Ellen D Semke

Qi Chen

Zheyong Yu

Zhenghong Zhang

Disclosed herein are glucosyltransferases with modified amino acid sequences. Such engineered enzymes exhibit improved alpha-glucan product yields and/or lower leucrose yields, for example. Further disclosed are reactions and methods in which engineered glucosyltransferases are used to produce alpha-glucan.

Engineered glucosyltransferases

An enzymatically produced soluble α-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble α-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble α-glucan fiber are also provided.

Enzymatic synthesis of soluble glucan fiber

An enzymatically produced soluble &#x3b1;-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble &#x3b1;-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble &#x3b1;-glucan fiber are also provided.

Recombinant bacteria having an improved ability to utilize sucrose are provided. These recombinant bacteria have nucleotide sequences encoding sucrose utilization polypeptides integrated into their genome between the yihP gene or its homolog and the yihO gene or its homolog. Additionally, methods of utilizing the recombinant bacteria to produce products such as glycerol and glycerol-derived products are provided.

Recombinant bacteria having improved sucrose utilization

Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria are described. Additionally, variant or recombinant bacteria comprising these variant sucrose transporter polypeptides, and methods of utilizing the bacteria to produce products such as glycerol and glycerol-derived products are provided.

Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria

Growing community of inventors

Wallingford, PA, United States of America

Jian Ping Lai