Mgi Tech Co., Ltd.

Berkeley Lights, Inc.

Mgi Holdings Co., Limited

University of California

Life Technologies Corporation

Illumina Corporation

Bruker Cellular Analysis, Inc.

Jian Gong

Yan-You Lin

Cheng Frank Zhong

Shifeng Li

Randall D Lowe, Jr

Justin K Valley

Shao Ning Pei

Liang Wang

Mark R Andersen

Michael C Pallas

Kornelija Zgonc

Marcin Sikora

Gordon A Janaway

Casey R McFarland

Haopeng Wang

Gothami Padmabandu

Ferrier Le

Frank Zhong

Gaetan L Mathieu

Helmy A Eltoukhy

Tarun Kumar Khurana

Sebastian Bohm

Robert C Kain

Min-Jui Richard Shen

Arash Jamshidi

Alexander J Mastroianni

AmirAli Talasaz

Eric Hans Vermaas

Jason M McEwen

Kenneth M Kuhn

Jodi Tsu-An Loo

George Sakaldasis

James Michael Osmus

J Tanner Nevill

Mark P White

Ming-Chiang Wu

Chang-Jin Kim

John A Tenney

Aathavan Karunakaran

Kevin T Chapman

I-Jong Lin

Troy A Lionberger

Gang F Wang

Richard L Lemoine

Gregory G Lavieu

Sz-Chin Steven Lin

Yiwen Ouyang

Sz-Chin Lin

Matthew E Fowler

Kevin D Loutherback

Anupam Singhal

Phillip J M Elms

Jason M Mcewen

Randall D Lowe

Jr Randall D Lowe

Casey Mcfarland

A method and device for displacing fluid from a reagent cartridge () into a microfluidic device () and for loading the fluid into the reagent cartridge (). The reagent cartridge () may include a cartridge body and a pipette array with pipette tips () to engage inlets of a microfluidics or other cartridge, wherein the pipette tips () correspond in position to the plurality of inlets () of the microfluidic device (). Fluid may be loaded into or displaced from the microfluidic device () by a system of plungers (). The reagent cartridge may alternatively include blisters () having fluid reservoirs and dispensing tips (), each dispensing tip () including a pathway () that is fluidly coupled to a blister (). The fluid may be displaced from or loaded into the blister () via the dispensing tip (). A deformable seal () may be overlaid on the blisters () to seal the volumes of fluid within the blisters (), and may be deformed to displace the fluid.

Reagent cartridges for in-vitro devices

An apparatus for forming a plurality of microdroplets () from a droplet () includes a substrate (), a dielectric layer () on the substrate () and having a plurality of hydrophilic surface regions () spaced apart from each other by a hydrophobic surface (), and a plurality of electrodes () in the dielectric layer (). The electrodes () are configured to form an electric field (E) across the droplet () in response to voltages provided by a control circuit () to move the droplet () across the dielectric layer () in lateral direction (F) while leaving portions of the droplet () on the hydrophilic surface regions () to form the plurality of microdroplets () on the hydrophilic surface regions ().

Apparatus and method for forming droplets having predetermined volume by electrowetting

Methods and systems for quantification of a target nucleic acid in a sample are provided. The method includes forming a plurality of discrete sample portions. Each of the plurality of discrete sample portions comprising a portion of the sample, and a reaction mixture. The method further includes amplifying the plurality of discrete sample portions to form a plurality of discrete processed sample portions. At least one discrete processed sample portion containing nucleic acid amplification reaction products. Fluorescence signals are detected from the at least one of the plurality of discrete processed sample portions to determine a presence of the at least one target nucleic acid. The method also includes determining the respective volumes of the plurality of the plurality of discrete processed sample portions, and estimating the number of copies-per-unit-volume of the at least one target nucleic acid in the sample. Estimating the number of copies-per-unit-volume is based on the number of discrete processed sample portions determined to contain the at least one target nucleic acid therein.

System and method for determining copies-per-unit-volume using PCR and flow control of droplets

A microfluidic device includes first and second substrate structures. The first substrate structure has a first substrate surface configured to receive one or more droplets. A plurality of electrodes configured to apply an electric field to the droplets. The second substrate structure has a second substrate surface facing the first substrate surface and spaced apart from the first substrate surface to form a fluid channel. The microfluidic device has a first heating element adjacent to the first substrate structure and disposed on an opposite side of the first substrate surface, and a second heating element adjacent to the second substrate structure and disposed on an opposite side of the second substrate surface. The microfluidic device further includes one or more temperature sensors disposed adjacent to the fluid channel between the first substrate structure and the second substrate structure.

Temperature control on digital microfluidics device

An apparatus for forming a plurality of microdroplets from a droplet includes a substrate, a dielectric layer on the substrate and having a plurality of hydrophilic surface regions spaced apart from each other by a hydrophobic surface, and a plurality of electrodes covered by the dielectric layer. The electrodes are configured to form an electric field across the droplet in response to voltages provided by a control circuit to move the droplet across the dielectric layer in a lateral direction while leaving portions of the droplet on the hydrophilic surface regions to form the plurality of microdroplets on the hydrophilic surface regions.

Method for manufacturing an apparatus for manipulating a droplet

An apparatus () including multiple biological chips () includes a substrate (), a first adhesive layer () disposed on the substrate (), a first biological chip () and a second biological chip () disposed on the first adhesive layer () and attached to the substrate () by the adhesive layer (). The apparatus () further includes a filler () disposed between the first biological chip () and the second biological chip (). The filler () includes a second adhesive layer () extending between a side surface () of the first biological chip () and a side surface () of the second biological chip (), the second adhesive layer () attaching the first biological chip () to the second biological chip (). The filler () also includes a surface layer () disposed over the second adhesive layer (). The surface layer () has a hydrophobic surface that is co-planar with a top surface () of the first biological chip () and a top surface () of the second biological chip ().

System and method for integration of biological chips

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

Wafer level sequencing flow cell fabrication

Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section having a dielectrophoresis configuration; systems that include microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the aqueous droplet; related kits; and methods of manipulating droplets within the microfluidic devices.

Microfluidic apparatus having an optimized electrowetting surface and related systems and methods

Multilayer electrical connection for digital microfluidics on substrates

A method for forming sequencing flow cells can include providing a semiconductor wafer covered with a dielectric layer, and forming a patterned layer on the dielectric layer. The patterned layer has a differential surface that includes alternating first surface regions and second surface regions. The method can also include attaching a cover wafer to the semiconductor wafer to form a composite wafer structure including a plurality of flow cells. The composite wafer structure can then be singulated to form a plurality of dies. Each die forms a sequencing flow cell. The sequencing flow cell can include a flow channel between a portion of the patterned layer and a portion of the cover wafer, an inlet, and an outlet. Further, the method can include functionalizing the sequencing flow cell to create differential surfaces.

Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

Methods, systems and kits for in-pen assays

Microfluidic devices having an electrowetting configuration and an optimized droplet actuation surface are provided. The devices include a conductive substrate having a dielectric layer, a hydrophobic layer covalently bonded to the dielectric layer, and a first electrode electrically coupled to the dielectric layer and configured to be connected to a voltage source. The microfluidic devices also include a second electrode, optionally included in a cover, configured to be connected to the voltage source. The hydrophobic layer features self-associating molecules covalently bonded to a surface of the dielectric layer in a manner that produces a densely-packed monolayer that resists intercalation and or penetration by polar molecules or species. Also provided are microfluidic devices having an electrowetting configuration that further include a section or module having a dielectrophoresis configuration; systems that include any of the microfluidic devices in combination with an aqueous droplet and a fluidic medium immiscible with the medium of the aqueous droplet; related kits; and methods of manipulating droplets, optionally containing micro-objects such as biological cells, within the microfluidic devices.

Water level sequencing flow cell fabrication

Growing community of inventors

Danville, CA, United States of America

Jian Gong