Jiangnan University

Shandong Runde Biotechnology Co., Ltd

Wuxi Vimicro Corporation

Jcet Group Co., Ltd.

Jiangsu Sujing Group Co., Ltd

Bright Dairy & Food Co., Ltd.

Nanjing University

Zgmicro Wuxi Corporation

Sujing Group Automation Instrument Equipment Corporation

Bloomage Biotechnology Corporation Limited

Taixing Dongsheng Bio-Tech Co., Ltd

Inner Mongolia Mengniu Dairy (Group) Co., Ltd.

Jian Chen

Guocheng Du

Jianghua Li

Long Liu

Jingwen Zhou

Xueqin Lv

Weizhu Zeng

Fang Fang

Song Liu

Zhen Kang

Xian Yin

Jieying Deng

Juan Zhang

Yanfeng Liu

Yuhong Feng

Meng Ning

Yang Gu

Feng Zhu

Hongwei Guo

Peng Jin

Changfeng Liu

Jianxing Lu

Jing Wu

Cheng Zhang

Yaojian Lin

Shuo Liu

Weidong Shen

David Xiaodong Yang

Fengfei Liang

Jiyong Sun

Zhijie Hu

Fuxin Sun

Sai Jin

Xiaodong Jiang

Qiuju Zhang

Wei Lu

Chen Deng

Jiangong Lu

Yaokang Wu

Taichi Chen

Xiaomei Cao

Jianwei Jiang

Jun Fang

Shasha Zhou

Tengfei Niu

Yongkun Lv

Weixin Zhao

Xueqing Chen

Jiran Zhang

Xifei Yang

Yang Wayne Wang

Lei Wang

Lingqia Su

Li Liu

Danfeng Yang

Min Han

Aidong Li

Dan Wu

Uwe Sauer

Jie Chen

Tao Yang

Chunmei Chen

Haitao Shi

David Xiao Dong Yang

Xiang Chen

Chuanwang Hu

Zhengxiong Zhou

Danyi Liao

Hannes Link

Zhu Jiang

Yue Feng

Yun Jiao

Jie Ruan

Hongye Cheng

Hongzhi Zhang

Hui Gao

Yang Song

Yawen Zhao

Ningzi Guan

Wenwen Ding

Xinyao Lu

Zhuolin Feng

Xueliang Qiu

Xiaolong Zhang

Danong Zhou

Li Sun

Bohan Zhang

Fei Yu

Yuan Qian

Zhengming Zhu

Xinrui Zhao

Xinzhe Mao

Peishan Yang

Xianhao Xu

Miao Wang

Xiaomin Dong

Qiang Ren

Jiabin Fang

Song Gao

Hengrui Zhou

The invention provides astrain for producing a human milk lipid substitute. By integrating a heterologous lysophosphatidic acid acyltransferase intoand knocking out its own natural lysophosphatidic acid acyltransferase, the content of palmitic acid (C16:0) at Sn-2 position of triacylglycerol produced byis increased, to synthesize a human milk lipid substitute. On this basis, a metabolic pathway related gene is knocked out, to further increase the content of human milk lipid substitute in the product. In the present invention, a human milk lipid substitute is de novo synthesized byfor the first time, in which the total fatty acid is 15% or more, and the relative content of C16:0 at Sn-2 position reaches about 60%.

strain for producing human milk lipid substitute and use thereof

Disclosed is recombinantfor producing L-tyrosine and application thereof, and belongs to the technical fields of genetic engineering and bioengineering. According to the present disclosure, genes aroP and tyrP are knocked out, expresses the endogenous gene yddG of, then heterologously expresses fpk from, expresses the endogenous genes ppsA and tktA of, and then expresses aroGand tyrA. Knocking out tyrR, trpE and pheA, so that the synthesis flux of L-tyrosine is increased. Finally, an endogenous gene poxB is knocked out to realize stable fermentation performance at high glucose concentration.

Recombinantfor producing l-tyrosine and application thereof

The present disclosure provides an automatic production line for manufacturing and processing plant protein meat, and belongs to the field of application of food equipment. The automatic production line for manufacturing and processing the plant protein meat comprises: an extrusion-expansion machine, plant protein meat raw material blocks, a bearing plate, a first manipulator, a storage rack, a first conveying device, a second manipulator, a second conveying device, a main console, a third conveying device, a shredding device, a seasoning adding and mixing device, a third manipulator, a recycling rack, a storage cabinet and a secondary console. The shredding device of the present disclosure shreds the plant protein meat raw material blocks by cooperative up-down staggered motion of double rows of clips, the plant protein meat raw material blocks in any shape can be shredded, and breakage of the raw material shreds in the shredding process can be avoided effectively; and by the seasoning adding and mixing device of the present disclosure, the shredded plant protein meat raw material blocks are added with seasonings and mixed with the seasonings. The present disclosure is simple in structure, facilitates manufacturing of the plant protein meat, and can efficiently produce and process the plant protein meat.

Automatic production line for manufacturing and processing plant protein meat

The present invention discloses a RamA transcription factor mutant for promoting the production of N-acetylglucosamine and use thereof. The mutant is obtained by mutating lysine at position 90 to asparagine and serine at position 92 to lysine in a parent having an amino acid sequence as shown in SEQ ID NO: 2. The present invention provides a genetically engineered strain that overexpresses the RamA transcription factor mutant and increases the production of N-acetylglucosamine. By overexpressing the transcription factor RamA that is involved in the regulation of carbon metabolism, the extracellular accumulation of N-acetylglucosamine is increased, with a maximum concentration reaching 31.5 g/L, which lays a foundation for further metabolic engineering ofto produce glucosamine. The method for constructing recombinantof the invention is simple, and convenient in use, and thus has good application prospects.

RamA transcription factor mutant for promoting production of N-acetylglucosamine and use thereof

The present invention provides an SIP package structure. The SIP package structure comprises a first module, a second module and a shielding assembly, wherein the first module and the second module are horizontally distributed or vertically stacked; electromagnetic sensitive frequencies of the first module and the second module are different; the shielding assembly comprises a first shielding structure covering the first module and a second shielding structure covering the second module; and at least part of the first shielding structure and/or at least part of the second shielding structure are/is disposed between the first module and the second module.

SIP package structure

The present invention provides a method for promoting N-acetylglucosamine synthesis by using the GlcN6P responsive element. In the present invention,BSGNY-P-glmS-P-GNA1 is used as a starting strain, in which a CRISPRi system regulated by GlcN6P responsive element is integrated into the genome to dynamically weaken the N-acetylglucosamine synthesis competitive pathway; a GlcN6P responsive promoter is used to regulate the expression of GNA1 on the plasmid to dynamically regulate the N-acetylglucosamine synthesis pathway; and the key gene alsSD involved in the acetoin synthesis pathway is knocked out. During fed-batch fermentation with this strain in a 15 L fermenter, the production of N-acetylglucosamine reaches 131.6 g/L and no by-product acetoin is accumulated, which lays a foundation for the production of GlcNAc by industrial fermentation.

Method for promoting n-acetylglucosamine synthesis by using GlcN6P responsive element

The present invention relates to an integrated package structure. The integrated package structure includes a main substrate, a first module, a second module, a cavity element and a large-size device, wherein the main substrate includes a first surface of the main substrate and a second surface of the main substrate opposite to each other; the first module and the second module are stacked; the first module and the second module which are stacked, the cavity element and the large-size device are horizontally arranged on the first surface of the main substrate, and are respectively electrically connected to the main substrate. Owing to this arrangement, the demand of a current integrated package structure for a further high-density, miniaturized, multi-dimensional and multi-demand layout design can be met.

Integrated package structure

The invention discloses a recombinantfor efficient synthesis of highly pure hyaluronic acid and oligosaccharides thereof, belonging to the technical field of bioengineering. The recombinantconstructed in the present invention can produce hyaluronic acid with a yield up to 40 g/L, and a crude product purity of 95%. Addition of exogenous hyaluronic acid hydrolase and optimization of the fermentation conditions results in hyaluronic acid oligosaccharides with specific molecular weight, and can further improve the yield of hyaluronic acid to 72 g/L. The invention lays a solid foundation for the efficient synthesis of highly pure hyaluronic acid by microorganisms, and the constructed recombinantis suitable for industrial production and application.

High-efficiency synthesis and high-purity hyaluronic acid, and recombinantfor oligosaccharide thereof

The present disclosure discloses a salt-reduced fermentation method for high-salt dilute-state fermented soy sauce, and belongs to the technical field of fermentation engineering. The present disclosure separates and screensJL-5 andJDF-2 which inhibit the growth of spoilage bacteria from low-salt soy sauce mash, and by changing the microbial fermentation process of high-salt dilute-state soy sauce, the strains obtained by screening are used for the fermentation of low-salt soy sauce mash. Without changing the flavor and quality of soy sauce, low-salt fermentation of the soy sauce is enabled to proceed normally, and the biogenic amine content is less than 100 mg L, and the number of spoilage bacteria in the soy sauce are reduced. The prepared low-salt soy sauce has an amino acid nitrogen content of greater than 1.2 g·100 mL, contents of various spoilage bacteria of less than 1.0×10CFU·g, a NaCl concentration of no more than 10 g·100 mL, and a sodium content of less than or equal to 420 mg·10 mL. Without the addition of preservatives, the quality stability is the same as that of soy sauce without salt reduction.

Salt-reduced fermentation method for high-salt dilute-state fermented soy sauce

The disclosure discloses construction of recombinant Saccharomyces cerevisiae for synthesizing carminic acid and application thereof and belongs to the technical field of genetic engineering and bioengineering. The disclosure obtains recombinantCA-B2 capable of synthesizing carminic acid by heterologously expressing cyclase Zhul, aromatase ZhuJ, OKS of Octaketide synthase 1, C-glucosyltransferase UGT2, monooxygenase aptC and 4'-phosphopantetheinyl transferase npgA in. The recombinantcan be used for synthesizing carminic acid by taking self-synthesized acetyl-CoA and malonyl-CoA as a precursor. On this basis, OKS, cyclase, aromatase, C-glucosyltransferase and monooxygenase relevant to carminic acid are integrated to a high copy site, which can remarkably improve the yield of carminic acid. The yield of carminic acid can be increased to 2664.6 &#x3bc;g/L by optimizing fermentation conditions, and the fermentation time is shortened significantly. Therefore, the recombinantplays an important role in the fields of cosmetics, textiles and food.

Construction of recombinantfor synthesizing carminic acid and application thereof

A control method of an intelligent sterility test pump, a sterilization method and its application. The control method includes monitoring the real-time current of a DC drive motor through a current detection module, to adjust the rotating linear velocity of a peristaltic pump head in real time; when the peristaltic pump head is in a state of pushing a liquid to be tested, reducing the rotating linear velocity of the peristaltic pump head; and when the peristaltic pump head is in an idling state, increasing the rotating linear velocity of the peristaltic pump head. A sterilization method includes the control method of the intelligent sterility test pump. Application of the sterilization method in preparation or detection of foods and medicines.

Control method of intelligent sterility test pump, sterilization method and application thereof

Disclosed is a flexible hydrogen sensor with ultra-high sensitivity and a wide range and a fabrication method therefor. The sensor includes a conductive electrode layer (), a sensing layer and a flexible substrate layer () in sequence from top to bottom. The sensing layer includes a MOfilm () and Pd nanoparticles (NPs) (), and the Pd NPs () are covered on the MOfilm (). A traditional metal oxide type hydrogen sensor and a quantum conductance-based hydrogen sensor are combined on a flexible polymer substrate by means of an atomic layer deposition (ALD) technology and a cluster beam deposition (CBD) technology, so as to obtain a flexible hydrogen sensor with ultra-high sensitivity, a wide range and excellent selectivity and lower working temperature.

Flexible hydrogen sensor with ultra-high sensitivity and wide range and fabrication method therefor

The disclosure discloses recombinantfor synthesizing e lacto-N-neotetraose yield. The recombinantis obtained by integrating two &#x3b2;-1,4-galactotransferase genes on a genome of a host bacterium168&#x394;amyE:P-lacY, P-lgtB, P-comK and exogenously expressing a &#x3b2;-1,3-N-glucosaminotransferase gene. Compared with a strain before transformation, the recombinantof the disclosure improves the yield of the synthesized lacto-N-neotetraose from 720 mg/L to 1300 mg/L, laying a foundation for further metabolic engineering transformation offor producing the lacto-N-neotetraose.

Recombinantfor synthesizing lacto-N-neotetraose and application thereof

The present invention provides a pyruvate-responsive biosensor and a construction method and use thereof. In the present invention, pyruvate-responsive biosensors with different dynamic ranges are successfully constructed by optimizing the PdhR binding sequence inserted on the P43 promoter and optimizing the insertion site, wherein the minimum increase in dynamic range is by 0.6 time, and the maximum increase is by 30.7 times. The pyruvate-responsive biosensors are useful in the precise control of the expression of each gene in the cell. Since pyruvate is a key metabolite of central carbon in the cells, these biosensors are capable of dynamically regulating the expression level of intracellular genes according to changes in the content of pyruvate in the cells, thereby achieving the dynamic control of intracellular metabolic flux. The pyruvate biosensor obtained in the present invention has a good specificity, and a response range to pyruvate of 10-35 nmol/g DCW.

Pyruvate-responsive biosensor, and construction method and use thereof

The disclosure discloses a genetically engineered strain for producing porcine myoglobin and fermentation and purification thereof, and belongs to the technical field of genetic engineering. The disclosure realizes efficient secretion and expression of porcine myoglobin by integrating the gene of porcine myoglobin in. On this basis, optimization of the medium and culture conditions of recombinantcan significantly increase the titer of porcine myoglobin, so that the titer can reach 285.42 mg/L under fermenter conditions. In addition, by creatively adding different concentrations of ammonium sulfate to fermentation broth step by step, the purity of myoglobin obtained by final concentration is up to 88.0%, and the purification rate is up to 66.1%. The disclosure realizes efficient expression and high purification of porcine myoglobin from various steps such as synthesis, fermentation and purification of porcine myoglobin, and provides broad prospects for industrial production of porcine myoglobin.

Genetically engineered strain for producing porcine myoglobin and food-grade fermentation and purification thereof

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Wuxi, China

Jian Chen