Tsinghua University

Capitalbio Corporation

Jiaming Ye

Wanli Xing

Guoqing Wang

Pinhong Wang

Dong Wang

Jing Cheng

Huafang Gao

Kaijun Zhao

Liqing Duan

Jinxiu Zhang

Xiumei Song

Kaihuan Zhang

In one aspect, a microfluidic device for multiple reactions is provided, which comprises a reaction channel comprising multiple reaction chambers connected to a closed chamber or an elastic balloon outside of the microfluidic device, wherein a wall of the closed chamber is an elastic membrane; and a control channel comprising an elastic side wall, wherein the intersections between the side wall of the control channel with the reaction channel form multiple pneumatic microvalves. In another aspect, a method for conducting multiple reactions using the microfluidic device is provided, which comprises: a) filling the reaction chambers with a sample; and b) applying pressure to the control channel to expand the elastic side wall of the control channel, wherein the expanded elastic side wall forms a pneumatic microvalve that separates the reaction chambers.

Microfluidic devices for multi-index biochemical detection

A hybridization system is provided, which comprises a biological chip having a substrate () with a probe dot matrix () and a cover plate () with at least two through-holes (), where a hybrid chamber () is formed between the substrate () and the cover plate (), at least two fluid channels () are interconnected respectively with the hybrid chamber () by the two through-holes (), and a fluid control device is interconnected with the fluid channels (). The biological chip hybridization system integrates hybridizing, cleaning and drying functions, uses power provided by the fluid control device as the drive force for promoting the liquid flow for automatically reciprocation flow in the fluid channels () and the chamber () to achieve a dynamic hybridization, thus improving the hybrid efficiency and uniformity, and achieving automatic control.

Biological chip hybridization system

An automatic injection device comprises at least an injection unit (). The said injection unit () is formed by sealing a cover plate layer () with hydrophilic surfaces and a microfluid layer (). The said cover plate layer () is provided with at least two through holes (). The said microfluid layer () is provided with a hollow-out hybridization chamber () and at least two hollow-out microfluid channels (). One end of each channel () is connected with the hybridization chamber (), and the other end is connected with a through hole () of the cover plate layer () respectively. Taking advantage of the hydrophilicity of the cover plate, the automatic injection device makes a solution automatically enter and fill the hybridization chamber () and the microfluid channels () by the driving force of liquid surface tension. The flow uniformity of sample solution in microarray chip is achieved by the structural design of the hybridization chamber () and the microfluid channels (). The automatic injection device has advantages of simple manufacture, easy operation, high hybridization efficiency, low sample cost, and automatic quantificational injection.

Automatic injection device for microarray chip and automatic injection hybridization microarray chip

An interface device for bio-chip at least comprises an interface unit, said interface unit consisting of instrument interface layer, fluid channel layer and sample interface layer. The instrument interface layer has at least one instrument interface. The fluid channel layer has one hollow-out fluid channel. The sample interface layer has at least one sample interface, and both ends of said fluid channel connect to the sample interface and the instrument interface respectively. The interface device separates sample solutions from the instrument interface by gas or liquid in the fluid channel, thus to avoid direct contacts between sample solutions and instrument interface which will cause pollution, and omitting cleaning processes after using instruments. With simple structure, easy operation and low cost, it applies to chemistry field, biology field and medical analysis field.

Interface device for bio-chip

Growing community of inventors

Beijing, China

Jiaming Ye