Jiangsu Trautec Medical Technology Co., Ltd.

Jiajia Li

Liping Wang

Song Qian

Pengfei Cheng

Chenming Qian

Huimin Liu

Wenwen Jiang

A recombinant collagen and a preparation method and use thereof, and in particular, a full-length collagen α1 chain produced through recombinant expression, and a preparation method and use thereof are provided. When a variant (which is denoted as α1 (I) M1) of a human type I collagen α1 chain (α1 (I) chain) and a variant (which is denoted as α1 (II) M6) of a human type II collagen α1 chain (α1 (II) chain) constructed in the present disclosure are produced through recombinant expression in, a main degradation product (a main degradation band) in substantially the same proportion as a target product (a target band) of a full-length α1 chain occurring during the recombinant expression of native full-length α1 (I) chain and α1 (II) chain is eliminated, and a yield of the target product is improved.

Recombinant collagen and preparation method and use thereof

A recombinant human type XVII collagen consists of an amino acid sequence shown in (A)or includes the amino acid sequence shown in (A), where A is a sequence set forth in SEQ ID NO: 2, a sequence undergoing an amino acid modification to a predetermined extent based on SEQ ID NO: 2, or a sequence that has more than 80% homology with SEQ ID NO: 2; n is an integer greater than or equal to 1; and A represents a basic unit, and when there are two or more basic units, the two or more basic units are identical or different and are directly connected in tandem through a peptide bond. In the present disclosure, it is confirmed that the recombinant human type XVII collagen can undergo efficient secretory and soluble expression in eukaryotic host cells such as().

Recombinant human type XVII collagen, and preparation method and use thereof

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Changzhou, China

Jiajia Li