Variagenics, Inc.

The General Hospital Corporation

Sequenom, Inc.

Other

Jia Liu Wolfe

Gregory Lawrence Verdine

Vincent P Stanton, Jr

Tomohiko Kawate

Brian Seed

Charles Allerson

Jeffrey Charles Olson

Glen S Cho

Vince P Stanton, Jr

Martin Zillmann

Chia-Iun Tsai

Chia-Lun Tsai

Colin W Dykes

Chai-Iun Tsai

The invention features compositions based on thioredoxin-like fold protein domains described as engineered thioredoxin-like fold proteins (ETRXs). These proteins include one or more artificially diversified thioredoxin-like fold protein domains; each domain may be originated from the same or different thioredoxin-like fold protein domains. Features of the invention also include methods for identifying and preparing an enriched composition of target binding, loop-diversified ETRXs with additional sequence variations to improve affinity, stability, selectivity, or solubility. The invention also features compositions of ETRXs substituted with prosthetic groups, polymers, proteins, nucleic acids, carbohydrates, metals, natural or synthetic small molecules and toxins.

Engineered thioredoxin-like fold proteins

The present invention provides methods and compositions for treating various diseases through selective killing of targeted cells using a combinatorial targeting approach. The invention features protoxin fusion proteins containing a cell targeting moiety and, a modifiable activation moiety which is activated by an activation moiety not naturally operably found in, on, or in the vicinity of a target cell. These methods also include the combinatorial use of two or more therapeutic agents, at minimum comprising a protoxin and a protoxin activator, to target and destroy a specific cell population.

Methods, compositions, and kits for the selective activation of protoxins through combinatorial targeting

The invention features recombinant exotoxins fromare for the therapeutic treatment of a variety of human diseases, particularly diseases characterized by an abundance or excess of undesired cells.

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The present invention relates to methods for the detection of polymorphisms by substituting a base-modified nucleotide for a natural nucleotide in a polynucleotide. The base-modified nucleotide renders the polynucleotide more susceptible to cleavage at the sites of its incorporation than site consisting of natural nucleotides. The fragments obtained are then analyzed to determine the presence or absence of a polymorphism.

Base-modified nucleotides and their use for polymorphism detection

The present invention relates to a method for genotyping a diploid organism to detect single nucleotide polymorphisms (SNPs) using modified nucleotides or nucleotide residues substituted with fluorescent groups.

Fluorescence-based genotyping

The present invention relates to methods for the detection of polymorphism in polynucleotides by using hybridization of fragments of segments of a polynucleotide suspected of containing a polymorphism with an oligonucleotide having a sequence complementary to a fragment identifying the polymorphism and subsequent detection of incorporated labels in the oligonucleotide-fragment duplex.

Method for identifying polymorphisms

The present invention relates to a method for genotyping a diploid organism by cleaving segments of two alleles such that 7-20 nucleotide fragments that contain a suspected polymorphic locus are produced and comparing the masses of those fragments.

Genotyping by mass spectrometric analysis of allelic fragments

The present invention is directed to novel base-modified nucleotides and methods for their use in the preparation and cleavage of modified polynucleotides.

Base-modified nucleotides and cleavage of polynucleotides incorporating them

The present invention relates to a method of synthesizing a polynucleotide with incorporation of a modified nucleotide.

Method for analyzing polynucleotides

The present invention relates to methods for the analysis of polynucleotides including detection of variance in nucleotide sequence without the need for full sequence determination, full sequence determination of a polynucleotide, genotyping of DNA and labeling a polynucleotide fragment during the process of cleaving it into fragments.

Growing community of inventors

Winchester, MA, United States of America

Jia Liu Wolfe