Institute of Nuclear Energy Research

National Pingtung University of Science & Technology

Jia-Baau Wang

Wen-Song Hwang

Gia-Luen Guo

Chiung-Fang Huang

Ting-Hsiang Lin

Yi-Feng Jiang

Teng-Chieh Hsu

Yo-Chia Chen

Wen-Hua Chen

Sheng-Shieh Chen

Tien-Yang Ma

Gia-Leun Guo

Yun-Peng Chao

Guan-Lin Yeh

Jeng-Chen Liu

Hau-Yu Wang

A method for producing xylitol by fermentation of lignocellulosic hydrolysates without detoxification is provided. By using the originally isolated yeastsp., xylose can be effectively converted into xylitol. The invention also provides thestrain having high furfural tolerance, and is capable to produce xylitol from various types of non-detoxified lignocellulosic hydrolysates, in which the overall utilization of xylose in hydrolysate can reach over 95%.

Method for producing xylitol from lignocellulosic hydrolysates without detoxification

A method for preparing a xylose-utilizing strain ofand theare introduced. The preferred recombinant strain contains multiple copies of integrated xylose metabolic genes, and can rapidly ferment xylose to produce ethanol from synthetic medium and lignocellulosic raw materials. The xylose-utilizing strain is applicable for the cellulosic ethanol production industry and brewing industry.

Method for preparing xylose-utilizing strain

A cellulose hydrolase and a gene thereof are obtained by screening a cDNA genomic library constructed withsp. Y102. The gene is 1071 base pairs long and comprises an open reading frame (ORF) for producing the cellulose hydrolase comprising 357 amino acids by translation. A transformed cell and a carrier carrying the gene are introduced. The gene is transferred toby transformation, such thatcan acquire activity of decomposing CMC, beta-glucan, and xylan. The cellulose hydrolase is multifunctional and is capable of decomposing cellubiose and directly decomposing fiber into glucose.

Cellulose hydrolase and gene thereof

Disclosed is a method for increasing the concentration of xylose in lignocellulosic hydrolysate. The method includes the step of extruding lignocellulosic material and mixing the lignocellulosic material with diluted acid and the step of conducting hot-water extraction on the mixture. Hence, the concentration of the xylose in the lignocellulosic hydrolysate can achieve a high concentration of 60 g/L, and the yield of the xylose can reach to 90%.

Method for increasing the concentration of xylose in lignocellulosic hydrolysate

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Taipei, Taiwan

Jia-Baau Wang