Genechem Inc.

Ji Young Yang

Won Min Seo

Sun Youp Kang

Jin Suk Woo

Sang Mi Lee

Dae Hee Kim

Byung Gee Kim

Byung-Gee Kim

Yun Hee Choi

Jae-Kyung Song

Dea Hee Kim

Tae Geon Gil

Sang Hee Shim

In Kang Hur

Young Soo Jung

Kyoung Soon Jang

The present invention relates to a method for preparing a sialic acid derivative characterized by performing both of a process for preparing CMP-N-acetylneuraminic acid using N-acetyl-D-glucosamine and a process for preparing the sialic acid (neuraminic acid) derivative that combines a sialic acid with a galactose derivative or a lactose derivative, together, in one reactor. According to the method for preparing a sialic acid derivative of the present invention, expensive cytidine 5'-monophosphate (CMP) is capable of being recycled in a reactor, such that an amount of the CMP introduced into the reactor may be reduced, and the sialic acid derivative is capable of being prepared at a significantly high efficiency by using cheap N-acetyl-D-glucosamine, and pyruvate as substrates.

Method for preparing sialic acid derivative

The present invention relates to a novel N-acetylglucosamine-2-epimerase and a method for preparing CMP-N-acetylneuraminic acid, more specifically, relates to a N-acetylglucosamine-2-epimerase derived fromNCTC 9343, and a method for preparing CMP-N-acetylneuraminic acid using said N-acetylglucosamine-2-epimerase. According to the present invention, CMP-N-acetylneuraminic acid can be produced economically in a large amount through a one-step reaction using cytidine monophosphate and N-acetyl-D-glucosamine which are inexpensive substrates.

N-acetylglucosamine-2-epimerase and method for producing CMP-neuraminic acid using the same

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Daejeon, South Korea

Ji Young Yang