Korea Institute of Science and Technology

Absology Co., Ltd

Ji Yoon Kang

Tae Song Kim

Youhee Heo

Min Cheol Park

Choong S Kim

Young Soo Kim

Youngsoo Kim

Soo Hyun Lee

Kyoseon Hwang

Jin Woo Lee

Hyung Joon Kim

Ick Chan Kwon

Jeong Hoon Lee

Yun Kyung Kim

Sangyun Kim

Kyeong Sik Shin

Jae-hoon Ji

Kyeong-Sik Shin

Jin Seok Kim

Byeong-kwon Ju

Hyewhon Rhim

Nakwon Choi

Jaesung Park

Kwang Meyung Kim

Eun Gyeong Yang

Rhokyun Kwak

Sang Yun Kim

In Chan Youn

Hyun Joon Shin

Jun Uk Chu

Hyewon Seo

Kui Won Choi

Han-Sang Cho

In Hye Lee

Jae Hoon Ji

Kang Sun Lee

Jun Kyo Suh

Yi Jae Lee

Bo Hoon Han

You Hee Heo

Sung Shin Ryu

Su Kyoung Chae

Myung-Sic Chae

Jung Hwan Kim

Siwoo Cho

The present invention relates to a method for isolating extracellular vesicles comprising: preparing a microfluidic chip in which at least two microfluidic channels are formed and inlets and outlets are formed at both ends of the channels, respectively; injecting at least one aqueous solution into the inlet of the microfluidic chip to form a microfluid; injecting a sample containing extracellular vesicles into the inlet of the microfluidic chip; and recovering the fluid from the outlet of the microfluidic chip.

Method for isolating extracellular vesicles

The present disclosure relates to a system for monitoring post-translational modification of protein using a biosensor with a gap, which performs with high reliability a diagnosis of a disease associated with a target protein for which impedance is measured, by measuring an impedance of a sample introduced into a sensor and calculating a change rate of the measured impedance, and to a method of manufacturing the biosensor used for the system.

System for monitoring post-translational modification of protein using bio-sensor with gap and manufacturing method for bio-sensor

Disclosed is a method for body fluid-based neurodegenerative disease diagnosis through high-sensitive immunoassay of aggregated proteins by photooxidation-induced amplification. The method according to the present disclosure provides an effect of quantitatively analyzing aggregated proteins in the form of oligomers or monomers which are present in trace amounts in a body fluid and measures normal or abnormal protein aggregation by detecting the aggregated proteins in the form of oligomers or monomers with high sensitivity by reaction of antibody-conjugated enzymes selectively bound to the aggregated proteins with substrates and photooxidation-induced amplification, thereby allowing accurate diagnosis of a neurodegenerative disease.

Detection of aggregated proteins associated with neurodegenerative disease using a photooxidation-induced amplification immunoassay

The biosensor includes a substrate, an electrode pattern positioned on the substrate, a passivation layer which is formed with a plurality of holes spaced apart from each other, and a bead positioned at one or more holes among the plurality of holes, and to which an antibody is attached, the electrode pattern includes a first electrode pattern part and a second electrode pattern part spaced apart from the first electrode pattern part, which has a same height as a height of the first electrode pattern part, and forms an electric field with the first electrode pattern part.

Ultra-highly sensitive electrochemical biosensor using beads and method for manufacturing the same

Disclosed is a method of quantifying a biomarker with high sensitivity using photo-oxidation induced amplification. The method includes performing an enzyme-substrate reaction of a measurement sample including an enzyme labeled on any one selected from among an antibody, an aptamer, and a nucleic acid specifically bound to the biomarker, optically measuring one or more optical properties selected from among amounts of color formation, light emission, and fluorescence of a product during a photo-oxidation induced amplification process while the product resulting from the enzyme-substrate reaction is continuously exposed to light to thus perform the photo-oxidation induced amplification process, indexing a time-varying pattern of the measured optical properties, and quantifying a concentration of the biomarker included in the measurement sample by comparing an index extracted during the indexing with an index of a reference sample.

Method of quantifying biomarker with high sensitivity using photo-oxidation induced amplification

A method for providing information relevant to the diagnosis of a neurodegenerative disorder. The method includes (i) obtaining a biological sample containing vesicles from a subject, (ii) measuring the level of amyloid beta in the biological sample using an antibody specific for the N-terminus of amyloid beta, and (iii) comparing the measured level of amyloid beta in the biological sample with the level of amyloid beta in a previously prepared control sample.

Methods for providing information relevant to diagnosis of neurodegenerative disorder

Provided are an extracellular vesicle collecting apparatus using an electrode and a porous membrane and a method for using the same. The extracellular vesicle collecting apparatus includes i) an upper frame in which a pair of through holes is disposed so that a buffer solution flows therethrough, ii) a buffer tube which is inserted in the through holes and in which the buffer solution flows, iii) a positive electrode disposed on the upper frame, iv) a porous membrane located below the upper frame, v) a spacer which is located below the porous membrane and has a hollow space disposed therein, vi) a plurality of guide tubes through which a blood flows in the hollow space, vii) a lower frame which is coupled to the upper frame to be opposite to the upper frame and receives the porous membrane and the spacer therein, and viii) a negative electrode provided below the lower frame. Extracellular vesicles contained in the blood are collected by the porous membrane.

Collecting apparatus for extracellular vesicles and method for using the same

The present invention relates to a method for diagnosing a disease using an analysis of oligomer of an abnormal aggregated protein includes: (1) preparing a body fluid sample including at least one of blood, blood plasma, blood serum, saliva, urine, tear, and mucus; (2) making a dilution of the body fluid sample; (3) using a biosensor to measure and detect an aggregated protein in the diluted body fluid sample; (4) analyzing a signal change of the biosensor caused by the dilution of the aggregated protein to determine a slope according to the dilution from the measurements; and (5) analyzing a proportion of the oligomer from the slope according to the dilution to make a diagnosis. The method uses a biosensor to measure the impedance and the protein concentration of blood and detects the slope according to the numerical value of the monomer and the oligomer to diagnose normal or abnormal protein aggregation or the associated diseases with more accuracy.

Method for diagnosing diseases through oligomer analysis of abnormally aggregated proteins

A nerve electrode that is inserted into a living body and that is configured to attach to nerves is provided. The nerve electrode that is inserted into a living body and that is configured to attach to nerves includes: i) a flexible substrate; ii) a plurality of electrodes that are separately positioned on the flexible substrate; and iii) an insulating layer that is positioned at a separation space of the plurality of electrodes and that insulates the plurality of electrodes. The plurality of electrodes include i) at least one linear electrode, and ii) a planar electrode that is separated from the linear electrode. An anti-inflammatory drug transfer layer is positioned on the planar electrode.

Nerve electrode provided with anti-inflammatory drug and method of manufacturing the same

The present invention relates to an apparatus and a method for fabricating a microcapsule, more particularly to an apparatus and a method for fabricating a microcapsule, which enable to encapsulate uniform cell number in a microcapsule through cell distribution, improve cell viability in the microcapsule through fluid exchange, and ensure uniform microcapsule size. The apparatus for fabricating a microcapsule according to the present invention uses a plurality of microchannels which are spatially connected with one another and are designed such that fluid flows through them in a particular direction, and comprises a cell supply unit which supplies a fluid mixture of cells and a cell dropletizing material; and a droplet forming unit in which a dropletization inducing fluid supplied from one of the plurality of microchannels joins with the fluid mixture of the cells and the cell dropletizing material to form a droplet.

Apparatus and method for fabricating micro-capsule

Disclosed are a system and a method for artificial nerve networking capable of restoring a damaged nerve and allowing selective detection, analysis, transmission and stimulation of a signal from the damaged nerve. The artificial nerve networking system according to an embodiment of the present disclosure includes: a first nerve conduit connected at one end of a damaged nerve; a second nerve conduit connected at the other end of the damaged nerve; and an artificial nerve networking unit electrically connected to the first nerve conduit and the second nerve conduit and recovering the function of the damaged nerve by transmitting and receiving a signal to and from the damaged nerve.

Artificial nerve networking system and method for functional recovery of damaged nerve

Disclosed is an apparatus and method for injecting liquid-drops of particle mixture liquid including a mixture of biological particles, affecting magnetic field, and having only positive particles, which are combined with magnetic responsive material, separated by magnetic force, and having negative particles, which are not combined with magnetic responsive material, precipitated by gravity.

Apparatus and method for separating biological particles using difference between gravity and magnetic force

Disclosed relates to a method and an apparatus for measuring fluorescence polarization FP in a lab-on-a-chip and, more concretely, to a method and an apparatus that measure quantitatively interactions between biomolecules and fluorescently labeled biomaterials and enzyme activities using the measurement of fluorescence polarization FP. The method and the apparatus of the invention provide rapid assays with minute amounts of samples using an automated device compared to conventional methods. Accordingly, the method and the apparatus of the invention can be usefully applied to the measurement of interactions between biomolecules and to the protease assays using a protein substrate.

Method and apparatus for measuring fluorescence polarization in lab-on-a-chip

In a cantilever sensor and a fabrication thereof, by forming piezoelectric films on the same surface, it is possible to sense various information by an electric measuring method. The cantilever sensor comprises a first silicon nitride film formed onto the top surface of a silicon substrate; a silicon oxide film formed onto the first silicon nitride film; a lower electrode formed onto the silicon oxide film; a first piezoelectric film and a second piezoelectric film formed onto the lower electrode, the second piezoelectric film formed out of contact with the first piezoelectric film; an upper electrode respectively formed onto the first and second piezoelectric films; a protecting film formed onto the silicon oxide film, the lower electrode, the first and second piezoelectric films and the upper electrode; a first and a second openings respectively formed on the protecting film on the upper electrode and the protecting film on the lower electrode; a first and a second contact pads respectively formed at the first and second openings; a T-shaped sensing portion formed at the end of a cantilever; a second silicon nitride film formed on the bottom surface of the silicon substrate.

Cantilever sensor and fabrication method thereof

Growing community of inventors

Seoul, South Korea

Ji Yoon Kang