Hanwha Chemical Corporation

Prestige Biopharma Pte. Ltd.

Ji Yong Yoon

Dong Eun Lee

Kyung Duk Moon

Dong Heon Lee

Byung Je Sung

Won Kyum Kim

Su Yeon Ryu

Jung Tae Lee

Young Woo Park

Ji Hyun Park

Jung Woo Lee

Jung Eun Baek

Myeoung Hee Jang

Yong Ho Ahn

Sang Kyung Park

Kyung Jae Kang

Jae Young Chang

So Young Choi

Yong Jin Kim

Eun Ho Hwang

Il Sun Lee

Kyo Eun Ahn

Jeong Won Youn

Jun Yong Park

Hyun Bo Shim

Provided are a method of preparing an antibody with a regulated, sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content, a method of preparing a high-quality population of antibodies by regulating the sugar content of the antibody to a desired content, and a population of antibodies prepared by the method. Further, provided is a method of regulating the antibody sugar chain content, the method including the step of culturing antibody-expressing cells in a medium including glycerol as an additive for regulating the antibody sugar chain content. Furthermore, provided is a medium composition, for regulating the antibody sugar chain content, the medium composition including glycerol as an additive for regulating the antibody sugar chain content. The preparation method of antibodies according to the present invention may be used to prepare a desired high-quality population of antibodies by regulating the sugar chain content of the antibody. Further, in terms of the development of biosimilars, the method of the present, invention may be used to regulate the sugar chain, content of antibodies, thereby preparing antibodies having high equivalence to a control drug. Since the sugar chain content may be regulated by a medium composition, the regulation method is easy and efficient in terms of time and cost, and therefore, widely applied to the fields of antibody preparation.

Method for preparing antibody through regulation of sugar content of antibody

The present invention relates to a method for preparing a population of antibodies to have high purity and high quality by removing antibody isoforms and impurities through the use of a cation exchange column, a hydrophobic interaction column, and an anion exchange column successively, without using a protein A column; and to a population of antibodies prepared by the above method.

Method of antibody purification

The present invention relates to a human monoclonal antibody that specifically binds to VCAM-1, and a therapeutic composition for the treatment of inflammatory disease or cancer comprising the same. The human monoclonal antibody according to the present invention shows a strong affinity to VCAM-1 expressed on human or mouse endothelial cell, effectively inhibits leukocyte adhesion to activated endothelial cells expressing VCAM-1, and shows a low immunogenicity, thereby being used for the treatment of cancer or inflammatory disease such as asthma, rhinitis, arthritis, multiple sclerosis, bowel disease, arteriosclerosis, myocardial infarction and transplant rejection.

Human monoclonal antibody that specifically binds to VCAM-1 and a composition for treating an inflammatory disease or a cancer comprising the same

The present invention relates to a human recombinant monoclonal antibody that specifically binds to human Vascular Cell Adhesion Molecule-1 (VCAM-1) to inhibit adhesion between leukocytes and activated endothelial cells and transmigration of leukocytes through the activated endothelial cells, and a prophylactic and therapeutic composition for inflammatory disease or cancer comprising the same. The human recombinant monoclonal antibody according to the present invention shows a strong affinity to VCAM-1 expressed on human endothelial cell, and effectively inhibits VCAM-1-mediated adhesion between leukocytes and activated endothelial cells and transmigration of leukocytes through the activated endothelial cells, thereby being used for the prevention and treatment of inflammatory disease such as asthma and arthritis, transplant rejection, cardiovascular disease, and cancer.

Human recombinant monoclonal antibody that specifically binds to VCAM-1 and inhibits adhesion and transmigration between leukocytes and endothelial cells

Growing community of inventors

Daejeon, South Korea

Ji Yong Yoon