Boston University

Purdue Research Foundation

Pulsethera, Inc.

Harvard College

Northwestern University

Other

Ji-Xin Cheng

Lu Lan

Delong Zhang

Pu-Ting Dong

Federico Capasso

Xiaoliang Sunney Xie

Jie Hui

Jiaze Yin

Chen Yang

Yifan Zhu

Nan Zheng

Yueming Li

Ying Jiang

Haonan Lin

Yi Zhang

M Selim Ünlü

Celalettin Yurdakul

Ali Shakouri

Lei Tian

Xiaoling Sunney Xie

Yeran Bai

D Kerry Maize

Cheng Zong

Alex Matlock

JIan Zhao

Carolyn Marar

Guangyuan Zhao

Daniela Matei

Yuying Tan

Junjie Li

Haonan Zong

Mohamed Seleem

Mingji Dai

Yao-Wei Huang

Pu Wang

Zhaoyi Li

Peng Lin

Yuhao Yuan

Hongli Ni

Qing Xia

Andreas Volkmer

Linli Shi

Chien-Sheng Liao

A wide-field microscopy system and method for imaging a sample include directing infrared light onto the sample to selectively heat the sample. Probe light is also directed onto the sample. An objective collects the probe light after it interacts with the sample. The collected probe light is detected at a detector. A relative distance between the objective and sample is adjusted to introduce an optical defocus enhancement to enhance detection of a change in detected probe light that is indicative of infrared absorption by the sample.

Widefield interferometric defocus-enhanced (WIDE) mid-infrared (MIR) photothermal microscopy

An example microscope includes a pump laser for providing a first illumination to a sample. A laser array provides a second illumination to the sample. The laser array may include a plurality of laser elements, each providing oblique illuminations to the sample. An illumination collecting source collects the first illumination and the second illumination from the sample. The illumination collecting source may capture transient 3D refractive index (RI) variations in the sample due to the first illumination and second illumination.

Bond-selective intensity diffraction tomography and uses thereof

A spectroscopy and microscopy system and method include a source of pulsed infrared light generating pulsed infrared excitation light for exciting a sample, such that the pulsed infrared light selectively heats the sample by absorption of the pulsed infrared light and allows the sample to cool. A source of probe light generates probe light for illuminating the sample. A detection element detects a signal indicative of selective heating of the sample by the excitation light. A processor receives the signal indicative of the selective heating of the sample, computes a time rate of local temperature rise in the sample to obtain instantaneous absorption in the sample, and, using the instantaneous absorption, generates a spectrum related to the sample.

Apparatus and method for microsecond-scale mid-infrared energy deposition (MIRED) spectroscopy and microscopy

Systems and methods implement of high-speed delay scanning for spectroscopic SRS imaging characterized by scanning a first pulsed beam across a stepwise reflective surface (such as a stepwise mirror or a reflective blazed grating) in a Littrow configuration to generate near continuous temporal delays relative to a second pulsed beam. Systems and methods also implement deep learning techniques for image restoration of spectroscopic SRS images using a trained encoder-decoder convolution neural network (CNN) which in some embodiments may be designed as a spatial-spectral residual net (SS-ResNet) characterized by two parallel filters including a first convolution filter on the spatial domain and a second convolution filter on the spectral domain.

High-speed delay scanning and deep learning techniques for spectroscopic SRS imaging

A short-wave infrared photothermal (SWIP) microscopy system and method for vibrational imaging of a sample generates shortwave infrared excitation light probe light. The excitation light and the probe light are combined to generate a combined beam, which is focused to generate a focused combined beam, which is directed onto the sample to obtain a SWIP signal generated by absorption-induced thermo-optic selective heating of the sample. The SWIP signal is collected through an aperture in a condenser and detected.

Apparatus and method for shortwave infrared photothermal (SWIP) microscopy

Systems and methods for detecting photothermal effect in a sample are described herein. In these systems and methods, a pump source is configured to generate a pump pulse train, a probe source is configured to generate a probe pulse train and is synchronized with the pump pulse train, and a camera collects the resulting data. The camera is configured to collect a first signal corresponding to a hot frame, wherein the hot frame includes visible probe beam as modified by a pump beam and a second signal corresponding to a cold frame, wherein the cold frame includes visible probe beam that has not been modified by a pump beam. A processor can subtract the second signal from the first signal to detect the photothermal effect.

Ultrafast chemical imaging by widefield photothermal sensing of infrared absorption

A system for neuromodulation includes a split-ring resonator (SRR) comprising a resonance circuit, the SRR being implantable in a cranial target site and a source of microwave signals, wherein the microwave signals are deliverable wirelessly to couple with the SRR to produce a localized electrical field, wherein the localized electrical field inhibits one or more neurons at the cranial target site with submillimeter spatial precision.

Wireless neuromodulation via microwave split ring resonator

A mid-infrared photothermal microscopy system images a sample. A mid-infrared optical source generates a mid-infrared beam which is directed along a first optical path to reach the substrate on a first side and heat the sample. A probe light source generates a probe light which is directed along a second optical path to reach the substrate on a second side and illuminate the sample. A first laser scanner is positioned along the first optical path and configured to rotate to redirect light and scan the sample with the mid-infrared beam. A second laser scanner is positioned along the second optical path and configured to rotate to redirect light and scan the sample with the probe light. The laser scanners each include at least one mirror driven to rotate such that the mid-infrared beam and the probe light scan the sample synchronously.

Video rate mid-infrared photothermal microscopy system using synchronized laser scanning

A stimulated Raman photothermal (SRP) microscope for imaging a sample. A first optical source omits an intensity-modulated pump beam. A second optical source omits an intensity-modulated Stokes beam. The Stokes beam is combined with the pump beam to form a combined beam. The combined beam is directed to the sample to induce a thermal effect caused by the stimulated Raman process. A third optical source emits a probe beam, the probe beam is directed to the sample. An optical detector detects modulation of the probe beam after modulation by the sample to measure an SRP signal.

Stimulated Raman photothermal microscope

Microscopic analysis of a sample includes a fluorescent dye disposed within the sample. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. An optical source generates a probe beam directed to impinge on the sample. A detector detects fluorescent emissions from the sample when the probe beam impinges on the sample. A data acquisition and processing system acquires and processes the detected fluorescent emissions from the sample to: (i) generate a signal indicative of infrared absorption by the sample, (ii) generate a signal indicative of temperature in the sample based on the signal indicative of infrared absorption by the sample, (iii) generate an image of the sample using the signal indicative of temperature in the sample.

Fluorescence-coded mid-infrared photothermal microscope

Microscopic analysis of a sample includes a system using dark-field illumination. A mid-IR optical source generates a mid-infrared beam, which is directed onto the sample to induce a temperature change by absorption of the mid-infrared beam. A visible light source generates a light illuminating the sample on a substrate and creating a scattered field and a reflected field along a collection path of the system. A pupil mask is positioned along the collection path to block the reflected field while allowing the scattered field to pass therethrough. A camera is positioned at an end of the collection path to collect the scattered field and generate a dark-field image of the sample.

Dark-field mid-infrared photothermal microscopy

Methods of the present invention comprise photoinactivation of catalase in combination with low-concentration peroxide solutions and/or ROS generating agents to provide antibacterial effects.

Bactericidal methods and compositions

Disclosed is an assay for determining resistance in a target cell or tissue to a therapy associated with cellular stress using chemical microscopy and high-throughput single cell analysis to determine functional metabolic alteration, including determining metabolic reprogramming in a target cell or tissue to a therapy associated with cellular stress, and methods of using the assays.

Device for detection of cellular stress

A display system includes an optical device configured according to constructive interference for a plurality of wavelengths at a focal length. The display system includes a fiber. The display system includes a controller configured to scan the fiber using a Lissajous scanning method to generate a display. The display can be disposed within a focal plane of the optical device. The controller is configured to modulate light intensity from the fiber. The controller can be configured to form a display image that passes through the optical device. The display system can include an optical combiner configured to reflect the display image from the optical device and form a virtual image. The optical device can be configured to magnify a display image from the display and form a virtual image.

Meta-optics for virtual reality and augmented reality systems

Systems and methods are provided for performing photothermal dynamic imaging. An exemplary method includes: scanning a sample to produce a plurality of raw photothermal dynamic signals; receiving the raw photothermal dynamic signals of the sample; generating a plurality of second signals by matched filtering the raw photothermal dynamic signals to reject non-modulated noise; and performing an inverse operation on the second signals to retrieve at least one thermodynamic signal in a temporal domain.

Nanosecond-scale photothermal dynamic imaging

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Newton, MA, United States of America

Ji-Xin Cheng