Mycogen Corporation

Abbott Laboratories Corporation

Valent Biosciences Corporation

Myocogen Corporation

Jewel M Payne

August J Sick

Kenneth Edwin Narva

George E Schwab

H Ernest Schnepf

Luis Foncerrada

Tracy Ellis Michaels

Raymond J Cannon

John B Randall

Jenny M Fu

Leslie A Hickle

M Keith Kennedy

Gregory A Bradfisch

George G Soares

Kendrick Akira Uyeda

Christine Julie Stalder

Heidi J Uick

Henry Meier

Pamela Gail Marrone

Chi-Li Liu

Hanne Gurtler

Mark Thompson

Annette S Petersen

Angela L Bagley

David A Cummings

David L Edwards

David O Brower

Theresa Galasan

Steve Stelman

Judy Muller-Cohn

Frank H Gaertner

Henry W Talbot

Angela L Ralph

Annette Schousboe Petersen

Harry E Schnepf

Robert C Everich

Michelle Burrascano

Theresa C Olson

Novelgenes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-activemicrobes. The DNA encoding thetoxins can be used to transform various prokaryotic and eukaryotic microbes to express thetoxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

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Novelgenes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-activemicrobes. The DNA encoding thetoxins can be used transform various prokaryotic and eukaryotic microbes to express thetoxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

Novel genes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-active microbes. The DNA encoding the toxins can be used to transform various prokaryotic and eukaryotic microbes to express the toxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins

Disclosed claimed are toxins produced by novel isolates designated . PS92J, . PS196S1, . PS201L1, and . PS201T6, which have dipteran and/or corn rootworm activity. Thus, the isolates, or mutants therof, can be used to control such pests. Further, claimed are novel genes encoding these &dgr;-endotoxins, which can be expressed in other hosts. Expression of the &dgr;-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Bacillus thuringiensis isolates and toxins

This invention concerns nematicidal proteins obtainable from isolates. The subject invention also provides various methods of using these proteins for controlling nematodes.

Nematicidal proteins

Bacillus thuringiensis isolated active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins

The invention relates to a mutant of,which produces a larger amount of crystal delta-endotoxin with a greater pesticidal activity as compared to the corresponding parental strain. The mutant may also have a larger crystal size as compared to the corresponding parental strain. The crystal delta-endotoxin produced by the mutant,will have an activity directed towards the same pest(s) as its parental,crystal delta-endotoxin. The invention further relates to a method for producing such a mutant, compositions comprising such a mutant as well as methods for controlling a pest(s) using these compositions.

Bacillus thuringiensis mutants which produce higher yields of crystal delta-endotoxin than their corresponding parental strains

This invention concerns genes or gene fragments which have been cloned from novel Bacillus thuringiensis isolates which have nematicidal activity. These genes or gene fragments can be used to transform suitable hosts for controlling nematodes.

Nematode-active toxins and genes which code therefor

Novel B.t. genes encoding toxins active against lepidopteran insects have been cloned from a novel lepidopteran-active B. thuringiensis isolate P515862. The DNA encoding the B.t. toxin can be used to transform various hosts to express the B.t. toxin.

Genes encoding lepidopteran-active toxins from Bacillus thuringiensis

Disclosed and claimed are approximately 130 kDa toxins produced by Bacillus thuringiensis PS201T6, which can be used to control pests. Further, claimed are novel genes encoding these .delta.-endotoxins, which can be expressed in other hosts. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts results in the control of susceptible insect pests in the environment of such hosts.

Polynucleotides encoding a 130 kDa pesticidal protein from Bacillus

Novel Bacillus thuringiensis genes encoding toxins which are active against lepidopteran insects have been cloned from novel lepidopteran-active B. thuringiensis microbes. The DNA encoding the B. thuringiensis toxins can be used to transform various prokaryotic and eukaryotic microbes to express the B. thuringiensis toxins. These recombinant microbes can be used to control lepidopteran insects in various environments.

Bacillus thuringiensis isolate active against lepidopteran pests, and

Novel Bacillus thuringiensis toxins with hymenopteran activity are described.

Bacillus thuringiensis toxins active against hymenopteran pests

The subject invention concerns Bacillus thuringiensis microbes with activity against select coleopteran pests, e.g., Diabrotica sp., Hypera sp., and various flea beetles. For example, the B.t. isolates of the invention are active against alfalfa weevils (AW, Hypera brunneipennis), rape flea beetles (RFB, Phyllotreta cruciferae), and corn rootworms (CRW, Diabrotica undecimpunctata undecimpunctata). Thus, these microbes can be used to control these pests. Further, genes encoding toxins active against these pests can be isolated from the B.t. isolates and used to transform other microbes. The transformed microbes then can be used to control susceptible coleopteran pests. In preferred embodiments, a gene from PS140E2 is used to transform plants so that the transformed plants are resistant to flea beetles of the genus Phyllotreta.

Bacillus thuringiensis isolates, toxins, and genes selectively active

Methods and compositions for the control of pests of the family Calliphoridae are described. Specifically, Bacillus thuringiensis (B.t.) isolates having anti-calliphorid activity are disclosed. Also described are recombinant hosts which express B.t. genes coding for pesticidal toxins. The B.t. isolates and recombinant proteins are shown to be useful in a method for controlling calliphorids including screw-worms and the sheep blowfly.

Materials and methods for the control of calliphoridae pests

Disclosed and claimed are novel Bacillus thuringiensis isolates which have lepidopteran activity. Thus, these isolates, or mutants thereof, can be used to control such insect pests. Further, genes encoding novel .delta.-endotoxins can be removed from the isolates and transferred to other host microbes, or plants. Expression of the .delta.-endotoxins in such hosts results in the control of susceptible insect pests in the environment of such hosts.

Protein toxins active against lepidopteran pests

Growing community of inventors

San Diego, CA, United States of America

Jewel M Payne