Biotica Technology Limited

Other

Pfizer Corporation

Jesus Cortes

Peter Francis Leadlay

James Staunton

Michael Stephen Pacey

Hamish Alastair Irvine McArthur

Hamish Alastair Irvine Mcarthur

A hybrid type I polyketide synthase gene typically containing a starter module and a plurality of heterologous extender modules is used to synthesise novel polyketides. It is preferably under the control of type II polypolyketide synthase promoter e.g. act I or

Method for producing hybrid polyketide synthases

A polyketide synthase ('PKS') of Type I is a complex multienzyme including a loading domain linked to a multiplicity of extension domains. The first extension module receives an acyl starter unit from the loading domain and each extension module adds a further ketide unit which may undergo processing (e.g. reduction). We have found that the Ksq domain possessed by some PKS's has decarboxylating activity, e.g. generating (substituted) acyl from (substituted) malonyl. The CLF domain of type II PKS's has similar activity. By inserting loading modules including such domains into PKS's not normally possessing them it is possible to control the starter units used.

Polyketides and their synthesis

Hybrid polyketide synthases and novel loading domains

A hybrid type I polyketide synthase gene typically containing a starter module and a plurality of heterologous extender modules is used to synthesize novel polyketides. It is preferably under the control of a type II polypolyketide synthase promoter e.g. act I of

Hybrid polyketide synthases combining heterologous loading and extender modules

Erythromycins, particularly ones with novel C-13 substituents R1 (e.g. C -C cycloalkyl or cycloalkenyl groups) are prepared by fermenting suitable organisms in the presence of R CO H. A preferred organism is preferably containing an integrated plasmid capable of directing synthesis of desired compounds.

Erythromycins and process for their preparation

Erythromycins, particularly with C-13 substituents R1 (e.g. C,-C,cycloalkyl or cycloalkenyl groups) are prepared by fermenting suitable organisms in the presence of R,CO,H. A preferred organism is,preferably containing an integrated plasmid capable of directing synthesis of desired compounds.

Growing community of inventors

Cambridge, United Kingdom

Jesus Cortes