3m Innovative Properties Company

Neogen Food Safety US Holdco Corporation

Jesse D Miller

Stephen B Roscoe

Stephanie J Moeller

Kurt J Halverson

Jason W Bjork

Myungchan Kang

Evan D Brutinel

Neil Percy

Minghua Dai

Shih-Hung Chou

Hsi-Chou Liu

Renee K Cline

Jerald K Rasmussen

Jimmie Rae Baran, Jr

Kannan Seshadri

Clinton P Waller, Jr

Douglas Eugene Weiss

James E Aysta

Steven P Swanson

Jeffrey A Lucas

Clinton P Waller

Assay devices are provided including a receptacle having a sample entry port; a plunger disposed within the receptacle; at least one reagent; a membrane attached to a first surface of the plunger; a wick providing capillary force; and a detection zone. The membrane includes a target conjugate zone in which affinity components are disposed. Capture compounds are immobilized in a detection zone, which is located between the target conjugate zone and the wick. A method of detecting a target analyte is also provided, including providing the assay device; providing a sample suspected to contain a target analyte; adding the sample onto the device; allowing the sample to travel along the membrane until the sample reaches the detection zone; immobilizing the target analyte through reaction of the target analyte with the capture compounds; reacting the immobilized target analyte with a reagent to generate a detectable signal; and detecting the generated signal.

Assay devices and method of detecting a target analyte

Compounds are provided that are either fluorogenic or fluorophoric. Compositions and articles that include the compounds are also provided. Additionally, methods of detecting a microorganism using the compounds are provided. The compounds are fluorinated and can be used advantageously under acidic conditions.

Fluorogenic or fluorophoric compounds and uses thereof

Compositions that comprise water, a first indicator reagent that can be converted by a first biological activity to a first biological derivative, and a plurality of particles are provided. The first indicator reagent can comprise a fluorogenic enzyme substrate having a fluorophore selected from the group consisting of umbelliferone, 7-aminocoumarin, &#x3b2;-naphthylamine, &#x3b2;-naphthol, fluorescein, resorufin, 9H-(1,3-dichloro-9,9-dimethyl acridin-2-one), rhodamine 110, a derivative of any of the foregoing fluorophores, and a combination of any of the foregoing fluorophores. The particles are capable of receiving and retaining the first biological derivative from an aqueous liquid. The first biological derivative can be indicative of a microorganism. The compositions further can comprise a gelling agent. Methods of using the compositions to detect the presence or absence of a microorganism in a sample are also provided.

Methods and compositions to detect a biological activity

A microbial detection article and methods of using the same, the article comprising: a base member comprising a self-supporting water impervious substrate with first and second generally opposed major surfaces; a filter assembly defining a filter assembly aperture therein, and having a composite filter body mounted across the filter assembly aperture; wherein the composite filter body comprises: a microporous membrane, and a water-absorbent layer in fluid communication with the microporous membrane; and a cover sheet.

Microbial detection article having a water-absorbent filter assembly

The disclosure includes: a coating composition, comprising a powdered cold-water-soluble gelling agent and surface-modified nanoparticles disposed in the powdered cold-water-soluble gelling agent; a coated film that includes a transparent film coated with the coating composition; and a device for growing microorganisms, including the coated film releasably attached to at least a portion of a body member that includes a self-supporting and water-proof substrate.

Device for rapidly detecting microorganisms

The disclosure provides an article for the detection of a microorganism in a liquid sample. The article comprises a microporous membrane and a barrier layer to selectively regulate the contact between the sample and a detection reagent. A method of use is also provided.

Microbial detection article

Methods for amplifying and detecting nucleic acids are described, as well as sets of 5' labeled oligonucleotides.

Dual oligonucleotide method of nucleic acid detection

Growing community of inventors

Hudson, WI, United States of America

Jesse D Miller