Fred Hutchinson Cancer Center

University of Washington

Jesse Bloom

Adam S Dingens

Allison Greaney

Neil P King

Daniel Ellis

Caelan Radford

Andrea Loes

Katharine H D Crawford

Tyler Starr

Cell-stored barcoded viral protein deep mutational scanning libraries are described. The libraries can be used to map resistance mutations to therapeutic treatments. The libraries can be used to predict viruses that become resistant to therapeutic compounds and/or may more easily evolve to infect new species. The libraries can also be used to more safely study dangerous viruses that normally require high safety biocontainment facilities. The libraries include features that allow efficient collection and assessment of informative data, obviating many bottlenecks of previous approaches.

Cell-stored barcoded deep mutational scanning libraries and uses of the same

Provided herein are compositions and methods comprising mutated coronavirus 'S' spike proteins or receptor binding domains thereof that have an increased expression level, yield and stability compared to its corresponding native or wild-type coronavirus spike protein under the same expression, culture or storage conditions. These mutated spike proteins can be used for generating a protein-based vaccine against one or more coronaviruses.

Stable coronavirus proteins and vaccine compositions thereof

Methods to create barcoded influenza viruses without disrupting the function of the viral proteins and the proper packaging of the viral genome segments are described. The barcoded influenza viruses can be used within deep mutational scanning libraries to map influenza resistance mutations to therapeutic treatments. The libraries can also be used to predict influenza strains that may become resistant to therapeutic treatments and/or more easily evolve to infect new species. The libraries include features that allow efficient collection and assessment of informative data, obviating bottlenecks of previous approaches.

Barcoded influenza viruses and deep mutational scanning libraries including the same

Growing community of inventors

Seattle, WA, United States of America

Jesse Bloom