Novo Nordisk A/s

Novozymes A/s

Jesper Vallentin Kierulff

Anders Hjelholt Pedersen

Tomas Tage Hansen

Yves De Grandpr&eacute;

Anthony James Knox

Ole Bill J&oslash;rgensen

The present invention deals with a process for oxidation of a hydroxy group of C and/or C and/or C and/or C and/or C and/or C of a sugar monomer of an oligo- or a polysaccharide comprising contacting, in an aqueous medium, the oligo- or the polysaccharide with a phenol oxidizing enzyme and an enhancing agent, whereby an oligo- or a polysaccharide with altered characteristics compared to the native oligo- or polysaccharide is created.

Modification of polysaccharides by means of a phenol oxidizing enzyme

A process for preparing tobacco, which process comprises the steps of treating a tobacco material with a phenol oxidising enzyme, such as by extracting tobacco with a solvent to provide an extract and a residue; and treating the extract with a phenol oxidising enzyme such as a laccase. An improved tobacco product having a reduced amount of phenolic compounds. This is an alternative or a supplement to a process in which the phenolic compounds are adsorbed onto the insoluble carrier polyvinylpolypyrrolidone (PVPP). In preferred embodiments, the process includes further steps of removing the oxidised phenolic compound, adding adsorbents such as bentonite; removing and/or inactivating the enzyme; and concentrating the extract. Preferred phenol oxidising enzymes are peroxidases and laccases. The thus treated extract is advantageously re-combined with the tobacco residue and further processed to provide a tobacco article for smoking.

Use of a phenol oxidizing enzyme in the treatment of tobacco

The present invention deals with a process for oxidation of a hydroxy group of C.sub.1 and/or C.sub.2 and/or C.sub.3 and/or C.sub.4 and/or C.sub.5 and/or C.sub.6 of a sugar monomer of an oligo- or a polysaccharide comprising contacting, in an aqueous medium, the oligo- or the polysaccharide with a phenol oxidizing enzyme and an enhancing agent, whereby an oligo- or a polysaccharide with altered characteristics compared to the native oligo- or polysaccharide is created.

Bleaching process comprising use of a phenol oxidizing enzyme, a

Bleaching process comprising use of phenol oxidizing enzyme, a hydrogen

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Roskilde, Denmark

Jesper Vallentin Kierulff