Ciba-geigy Corporation

Novartis Finance Corporation

Syngenta Investment Corporation

Jerzy Paszkowski

Ingo Potrykus

Thomas Hohn

Barbara Hohn

Vaclav Mandak

Michael William Saul

Raymond Douglas Shillito

Raymond D Shillito

Gabor Lazar

Hideaki Shinshi

Isabelle Rauseo

A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, direct to plant cells from which genetically transformed plants can subsequently be derived.

Transformation of hereditary material of plants

Transformation of hereditary material of Brassica plants and cells

A novel process for the direct transfer of foreign genes to plant genomes is described. The novel process comprises placing a gene under the control of plant expression signals and transferring it, by contact with protoplasts without the aid of natural systems for infecting plants, directly to plant cells from which genetically transformed plants can subsequently be derived.

A process for the maintenance and proliferation of defective, non-infectious viruses or virus genomes, comprising mutations, i.e. deletions, substitutions, insertions and new rearrangements of the viral genes or combinations of the virus DNA with heterologous genetic material in proliferating plant material and for the regeneration of whole plants containing stably integrated into their genome said defective, non-infectious virus DNA.

Process for the maintenance and proliferation of defective

The invention describes a process for culturing proliferating aggregates of plant cells, which comprises (a) uniformly plating isolated protoplasts, or isolated cells regenerated from protoplasts, in or on an agarose-solidified culture medium, and/or (b) cutting this pretreated and solidified culture medium into segments, transferring said segments to a liquid nutrient solution and continuing culturing in both cases until the cell aggregates have attained the desired size.

Propagation process employing agarose medium

Growing community of inventors

Zurich, Switzerland

Jerzy Paszkowski