Boston University

Ambergen, Inc.

Intelligent Bio-systems, Inc.

Qiagen Sciences, LLC

Isoplexis Corporation

Qiagen Waltham, Inc.

Other

Qiagen Gmbh

Qiagen Instruments Ag

Jerzy Olejnik

Kenneth J Rothschild

Cheng-Yao Chen

Steven Jeffrey Gordon

Sanjay M Sonar

Sadanand Gite

Edmund Golaski

Thomas Hagerott

Mong Sano Marma

Ilia Korboukh

Evan Guggenheim

Edyta Krzymanska-Olejnik

Mark J Lim

Visalakshi Visalakshi

Sergey Mamaev

Dirk Zimmermann

Michel Georges Perbost

Martina Werner

Phillip Alan Veatch

Angela DeLucia

Steve Gordon

Thomas William Schoenfeld

Visalakshi Meyyappan

Ryan Charles Heller

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Methods and compositions for incorporating nucleotides

The invention provides methods and compositions, including, without limitation, algorithms, computer readable media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. A plurality of smaller flow cells is employed, each with a relatively small area to be imaged, in order to provide greater flexibility and efficiency.

Methods and devices for sequencing nucleic acids in smaller batches

The present invention relates to a polymerase enzyme from 9° N with improved ability to incorporate reversibly terminating nucleotides. The enzyme comprising mutations in the motif A region. The invention also relates to methods of using such enzymes as well as a kit with such polymerases.

Polymerase enzyme from 9°N

The present invention provides methods, compositions, mixtures and kits utilizing deoxynucleoside triphosphates comprising a 3'-O position capped by a group comprising methylenedisulfide as a cleavable protecting group and a detectable label reversibly connected to the nucleobase of said deoxynucleoside. Such compounds provide new possibilities for future sequencing technologies, including but not limited to Sequencing by Synthesis.

Methods of using nucleotide analogues

Modular flow cells, devices with modular flow cells, and methods of sequencing using modular flow cells, as well as systems and kits including modular flow cells, are described, permitting sequencing wherein less than the full capacity for sequencing is desired.

Modular flow cells and methods of sequencing

Methods for synthesizing nucleotide analogues with disulfide linkers

The invention relates to methods, compositions, devices, systems and kits as described including, without limitation, reagents and mixtures for determining the identity of nucleic acids in nucleotide sequences using, for example, sequencing by synthesis methods. In particular, the present invention contemplates the use of polyphenolic compounds, known as antioxidant additives, to improve the efficiency of Sequencing-By-Synthesis reactions. For example, gallic acid (GA) is shown herein to be one of many exemplary SBS polyphenolic additives.

Polyphenolic additives in sequencing by synthesis

Nucleotide analogues

Methods and compositions for enriching a population of particles containing an analyte are disclosed. In one embodiment, enrichment beads are used that are larger in size than the beads used for amplification. A separation device is employed that can retain larger beads with bound amplified beads. The technique finds many uses, including enriching for beads with clonally amplified template, which can be used in a variety of assays, including nucleic acid sequencing.

Analyte enrichment methods and compositions

The invention provides methods and compositions, including, without limitation, algorithms, computer readable Media, computer programs, apparatus, and systems for determining the identity of nucleic acids in nucleotide sequences using, for example, data obtained from sequencing by synthesis methods. The methods of the invention include correcting one or more phenomena that are encountered during nucleotide sequencing, such as using sequencing by synthesis methods. These phenomena include, without limitation, sequence lead, sequence lag, spectral crosstalk, and noise resulting from variations in illumination and/or filter responses.

Growing community of inventors

Brookline, MA, United States of America

Jerzy Olejnik