University of Texas System

Biospectrum, Inc.

Regen Therapeutics Plc

Jerzy Alexander Georgiades

Marian L Kruzel

Thomas K Hughes, Jr

G John Stanton

Istvan Boldogh

Antoni Polanowski

Jerzy Gumulka

Tadeusz Wilusz

Marian Kruzel

The invention relates to the purification of peptides from colostrum. The method involves the addition of an alcohol such as methanol or ethanol to the mixture in order to form an alcohol phase rich in the peptides, and a precipitate. The peptide-rich alcohol phase is subsequently recovered and subjected to further fractionation. The invention is particularly useful in the purification of colostrinin from colostrum.

Purification of peptides from colostrum

The present invention provides methods that utilize compositions containing colostrinin, a constituent peptide thereof, an active analog thereof, and combinations thereof, as modulators of intracellular signaling molecules, for example.

Use of colostrinin, constituent peptides thereof, and analogs thereof as modulators of intracellular signaling molecules

The present invention discloses a use of colostrinin, a constituent peptide thereof, and/or an analog thereof as an immunological regulator and as a blood cell regulator in animals including humans.

Use of colostrinin, constituent peptides thereof, and analogs thereof for inducing cytokines

A process for the purification of human interferon alpha involving a three-step chromatography sequence under highly dissociating conditions is disclosed. The purification method involves a first step of loading crude interferon alpha onto a glass sorbent column to adsorb interferon alpha. The adsorbed interferon alpha is then eluted from the glass sorbent using a hydrophobic electrolyte solution comprising tetramethyl ammonium chloride, NaCl and propylene glycol. Thereafter, in a second step, the eluted interferon is subjected to SEPHACRYL S-200 molecular sieving chromatography under highly dissociating conditions and the fractions corresponding to molecular weights ranging from about 10,000 to about 40,000 are collected. In a third step, the collected fractions are then passed thorugh a column Zn++ chelating resin. The interferon alpha passes through unadsorbed to the Zn++ chelating resin. The resulting solution is a highly purified interferon alpha which preserves the apparently native isospecies compositions. Also disclosed is a method to enhance overall interferon alpha recovery which involves treating crude or partially purified interferon alpha solutions with a non-ionic detergent such as, for example, TRITON X-100, TWEEN 20, or NONIDET P-40.

Purification method for alpha interferon

A process for producing unique human interferon gamma and interleukin-2 by chromatographic fractionation utilizing ion exchange and metal chelate chromatography. The interferon gamma and interleukin-2 are purified from crude interferon obtained by mitogen induction of human white blood cells.

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Houston, TX, United States of America

Jerzy Alexander Georgiades