R.j. Reynolds Tobacco Company

Other

Jerry Wayne Marshall

Serban C Moldoveanu

Daniel Verdin Cantrell

Anthony Richard Gerardi

Marvin Glenn Riddick

Annett Milling

Michael F Davis

Michael Francis Dube

Dwayne William Beeson

Luis Monsalud

Alton Busbee

Susan Marlene Adams

Jo Ann Hill Hart

Barry Smith Fagg

Frank Kelley St Charles

Darlene Madeline Lawson

Gong Chen

Huamin Gan

Robert Reinbold

Wayne Allen Scott

Cheryl Cooper Scott

Jo A Hart

Ernest L Cerny, Iii

Keisha Renee Willingham

Anthony A Gerardi

Jo Ann Hart

A smokeless tobacco pastille configured for insertion into the mouth of a user is provided. The smokeless tobacco pastille can include (i) a tobacco material present in an amount of less than about 40 dry weight percent, based on the total dry weight of the pastille; (ii) at least one natural gum binder present in an amount of at least about 25 dry weight percent; and (iii) a plurality of sugar alcohols present in a total amount of at least about 40 dry weight percent, the predominant component of the plurality of sugar alcohols being isomalt or erythritol, wherein the shape of the smokeless tobacco pastille is moldable in the oral cavity.

Smokeless tobacco pastille

Methods and compositions are provided for expressing an endolysin in a bacterium or in a tobacco plant or plant part. Expression of the endolysin can reduce the total bacterial load on the plant and in tobacco products produced from the plant. Accordingly, the level of tobacco specific nitrosamines (TSNA) in a cured tobacco product can be decreased when the tobacco leaves used in the product were harvested from a plant expressing endolysin or when bacteria modified to express endolysin is used to treat the tobacco. Thus, bacteria and tobacco plants and plant parts are provided that express an endolysin. Further provided are methods for decreasing the microbial load on a tobacco plant and in a tobacco product by applying a solution of bacteria modified to express an endolysin or by modifying a tobacco plant to express endolysins. Smokeless tobacco products including such modified tobacco plant parts are also provided.

Methods and compositions for reducing bacterial load in tobacco

A smokeless tobacco product configured for insertion into the mouth of a user of the product is provided, the smokeless tobacco product including a dissolvable or meltable base composition admixed with a tobacco material and a botanical material, wherein the botanical material is present in an amount of at least about 0.1% of the total dry weight of the smokeless tobacco product. The botanical material and the smokeless tobacco product can be characterized based on antioxidant content using the ORAC index or the FRAP index. An exemplary product has an ORAC index value of at least about 20 (&#x3bc;mol TE)/g or a FRAP index value of at least about 50 (&#x3bc;mol/Fe)/g.

Smokeless tobacco composition incorporating a botanical material

A method of modifying the content of certain chemical compounds in tobacco materials is provided, the method including treatment of a tobacco plant or portion thereof with at least one enzyme. For example, the method may modify the content of tobacco smoke toxicant precursors in tobacco materials, which can result in a modification in toxicant production when the tobacco material is exposed to elevated temperatures. The type of tobacco plant or portion thereof treated according to the invention can be, for example, a tobacco seed, a tobacco seedling, an immature live plant, a mature live plant, a harvested plant, or a plant derivative. Smoking articles and other tobacco products including such enzyme-treated tobacco materials are also provided.

Method for treating tobacco plants with enzymes

A method of preparing a smoke-treated tobacco material is provided, the method including the steps of: (i) subjecting a tobacco material to smoke to give a smoke-treated tobacco material with modified taste characteristics; and (ii) incorporating the smoke-treated tobacco material into a smokeless tobacco product. The invention also provides a smokeless tobacco product incorporating a smoke-treated tobacco material. The smokeless tobacco product may be, for example, a moist snuff tobacco product.

Smoke treatment

Methods of modifying the tobacco-specific nitrosamine content of a tobacco material are described herein. One exemplary method comprises contacting a tobacco material with a composition comprising salt, sugar, enzyme, lactic acid bacteria, yeast, or a combination thereof to reduce the total bacterial content; curing the tobacco material; and fermenting the tobacco material in the presence of one or more microorganisms. The method can provide a fermented tobacco material having a tobacco-specific nitrosamine content that is reduced relative to a fermented tobacco material that has not been subjected to the disclosed method steps. In certain embodiments, the tobacco-specific nitrosamine content of the fermented tobacco material is no more than that of the cured tobacco material. Tobacco-containing products including such treated tobacco materials are also provided.

Treatment of tobacco

Disclosed is a process for inhibiting formation of nitrosamines by bacteria in tobacco, comprising contacting the tobacco with a substance comprising a plurality of bacteriophages capable of lysing bacteria that form nitrosamines. Disclosed also is a tobacco product possessing a diminished concentration of nitrosamines.

Process for inhibiting formation of nitrosamines

A method of curing tobacco. The method comprises the steps of contacting a tobacco material with a curing additive comprising one or more salts and curing the tobacco material under conditions and temperature for a time sufficient to produce cured tobacco wherein the amount of the one or more salts contacted with the tobacco is at least 5 percent of the dry weight of the cured tobacco.

Tobacco products and processes

A method of modifying the content of certain bacteria in uncured tobacco material is provided, the method including contacting an uncured tobacco material with a treatment solution, wherein the treatment solution is selected from the group consisting of: (i) a solution comprising salt, sugar, or a combination thereof; (ii) a solution comprising one or more enzymes; and (iii) a solution comprising one or more probiotics, wherein said contacting provides a treated tobacco material having a reduced total bacterial content following harvest. Also provided is a method of reducing TSNA content, the method including growing a tobacco plant in soil treated with a chloride source; harvesting the tobacco plant; and curing the harvested tobacco plant to give a cured tobacco material, providing a treated, cured tobacco material having a TSNA content that is reduced as compared to a cured tobacco material that has been grown in soil not treated with a chloride source. Smoking articles and smokeless tobacco products including such treated tobacco materials are also provided.

Modification of bacterial profile of tobacco

Growing community of inventors

Stokesdale, NC, United States of America

Jerry Wayne Marshall