Mycogen Plant Science, Inc.

The Upjohn Co.

Cornell Research Foundation Inc.

Seminis Vegetable Seeds, Inc.

University of Toledo

Lubrizol Genetics, Inc.

Asgrow Seed Company LLC

Mycoen Plant Science, Inc.

Jerry L Slightom

David A Tepfer

Dennis Gonsalves

John D Kemp

Timothy C Hall

Paula P Chee

Hector D Quemada

Dennis W Sutton

Stephen L Goldman

Anne C Graves

John F Reynolds

Kim J Carney

Maury L Boeshore

David M Tricoli

J Russell McMaster

David D Gleason

Chris Kearney

Norimoto Murai

Brigitte L'hostis

Paul T Frey

Lawrence D Jones

The present invention relates to the coat protein genes of Papaya Ringspot Virus Strain papaya ringspot (PRV-p), Watermelon Mosaic Virus II (WMVII), and Zucchini Yellow Mosaic Virus (ZYMV); to expression vectors which contain a coat protein gene for PVP-p, WMVII or ZYMV, and, additionally, the necessary genetic regulatory sequences needed for expression of a gene transferred into a plant; to bacterial or plant cells which are transformed with an expression vector containing the PVP-p, WMVII or ZYMV coat protein genes; to transgenic plants which are produced from plant cells transformed with an expression vector containing the coat protein gene from PVP-p, WMVII or ZYMV; and to a process of producing transgenic plants which have increased resistance to viral infection.

Potyvirus coat protein genes and plants transformed therewith

NIa protease genes of papaya ringspot virus strains FLA.83 W and USA P-type (HA attenuated) strain are provided.

Papaya ringspot virus protease gene

The sequence of the T.sub.L -DNA of Ri plasmids found in Agrobacterium rhizogenes strains HRI and A4 is disclosed. Sixteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found, five of which were observed to be transcripted in a developmentally and phenotypically regulated manner. The use of promoters and polyadenylation sites from pRi T.sub.L -DNA to control expression of heterologous foreign structural genes is taught, using as examples the structural genes for Phaseolus vulgaris storage protein (phaseolin), P. vulgaris lectin, a sweet protein (thaumatin), and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

Ri T-DNA promoters

A DNA fragment which encodes the coat protein of cucumber mosaic virus strain c (CMV-C), the method of preparing it, its use to prepare transgenic plants and transgenic plants containing it are provided.

Cucumber mosaic virus coat protein gene

The invention provides genetically modified plant cells having a plant structural gene introduced and expressed therein under the control of a T-DNA promoter. In particular, the invention provides genetically modified plant cells having the phaseolin gene introduced and expressed therein under the control of a T-DNA promoter. Also provided are novel strains of bacteria containing and replicating T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter. Further, the invention provides novel plasmids having the ability to replicate in E. coli and comprising T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter.

Plant structural gene expression

The phaseolin structural gene and promoter are disposed. Also disclosed are plasmids, bacteria, plant cells, plant tissue and plants containing the phaseolin promoter. The promoter finds use in the expression of heterologous plants genes, including the phaseolin gene, in transformed plant cells and plants.

Plant gene expression

RI T-DNA Promoters

A non-tissue culture process using Agrobacterium-mediated vectors to produce transgenic plants from seeds of such plants as the common bean and soybean.

Agrobacterium mediated transformation of germinating plant seeds

RI T-DNA promoters

The coat protein gene of cucumber mosaic virus strain WL (CMV-WL), the method of preparing it, its use to prepare transgenic plants and transgenic plants containing it are provided.

T-DNA promoters

The present invention relates to a recombinant multigene comprising a plurality of structural genes and a plurality of DNA sequences which encode peptide linkers. In the present invention, one of the structural genes encodes a protease, the DNA sequences encoding the peptide linkers are adjacent to the DNA sequences which encode the structural genes and the peptide linkers contain an amino acid sequence which the protease recognizes as a proteolytic cleavage site. The present invention additionally relates to transgenic plants which contain such a recombinant multigene transgene, host cells transformed with such a recombinant multigene, and transgenic animals which contain such a recombinant multigene transgene. Furthermore, the present invention relates to a method of producing a plurality of polypeptides in a host by incorporating and expressing in the host a such recombinant multigene.

Plant potyvirus expression vector with a gene for protease

The invention provides genetically modified plant cells having a plant structural gene introduced and expressed therein under the control of a T-DNA promoter. In particular, the invention provides genetically modified plant cells having the phaseolin gene introduced and expressed therein under the control of a T-DNA promoter. Also provided are novel strains of bacteria containing and replicating T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter. Further, the invention provides plasmids having the ability to replicate in E. coli and comprising T-DNA which has been modified to contain an inserted plant structural gene under the control of a T-DNA promoter.

Growing community of inventors

Kalamazoo, MI, United States of America

Jerry L Slightom