The State University of New York

Jeronimo Cello

Eckard Wimmer

Steffen Mueller

Bruce Futcher

John Robert Coleman

Steve Skiena

Dimitris Papamichail

Aniko V Paul

Hidemi Toyoda

Jiang Yin

This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

Attenuated viruses useful for vaccines

Methods of making modified viral genomes

A novel and stable attenuated poliovirus, which replicates in neuroblastoma cells, is produced by engineering an indigenous replication element (cre), into the 5' non-translated genomic region and inactivating the native cre element located in the coding region of 2C (mono-crePV). The stably attenuated poliovirus replicates in a neuroblastoma model (Neuro-2atumors) expressing CD155, the poliovirus receptor, and is effective for oncolytic treatment and cure of solid tumors, such as neuroblastoma.

Attenuated poliovirus

Growing community of inventors

Port Jefferson, NY, United States of America

Jeronimo Cello