Ventana Medical Systems, Inc.

University of Illinois

Jerome W Kosmeder, Ii

Christopher Bieniarz

Mark Lefever

Michael Patrick Farrell

Eric J May

Donald D Johnson

Julia Ashworth-Sharpe

Zhanna Zhilina

Casey A Kernag

Larry E Morrison

Brian Daniel Kelly

Adrian E Murillo

Jennifer Wong

Chol Steven Yun

Michael Otter

William Kenneth Day

John M Pezzuto

Benjamin C Stevens

Stacey Stanislaw

Nelson Alexander

Nathan Crum

Taylor J Shingler

Anne Pedata

Joseph M Jakubowski

Ze-Qi Xu

Noemi Sebastiao

Nian E Zhou

Penny Towne

James Grille

Linda Willoughby Kivi

Miriam Elaine Goldsmith

Pamela Wirth

Thomas M Grogan

Fabien Gaire

Paula M Rodgers

Richard C Moon

Alexandra Dea Nagy

Hiroaki Nitta

Robert M Moriarty

Lynda Li Song

Richard Gniewek

Megan Lehrkamp

Mary Padilla

Automated systems to make target compounds from slide stainer waste streams inactive utilizing advanced oxidation processes are described herein. Advanced oxidation processes are promoted by UV irradiation and further accelerated by use of radical initiators, such as hydrogen peroxide. The automated systems further include mechanisms for segregating components of the waste streams.

Passive, gravity-driven system for treatment of an effluent in a diagnostic system

Disclosed embodiments concern a composition comprising DAB chromogen, and/or derivative thereof, a stabilizer, and polymer capable of preventing or reducing DAB precipitation relative to a composition that does not comprise the polymer. Also disclosed herein is a method for using the disclosed composition and embodiments of a kit.

Polymer stabilization of chromogen solutions

Disclosed are compositions and methods for inactivating one or more enzymes in a biological sample.

Compositions and methods for simultaneous inactivation of alkaline phosphatase and peroxidase enzymes during automated multiplex tissue staining assays

Disclosed herein are embodiments of a signaling conjugate, embodiments of a method of using the signaling conjugates, and embodiments of a kit comprising the signaling conjugate. The disclosed signaling conjugate comprises a latent reactive moiety and a chromogenic moiety that may further comprise a linker suitable for coupling the latent reactive moiety to the chromogenic moiety. The signaling conjugate may be used to detect one or more targets in a biological sample and are capable of being covalently deposited directly on or proximally to the target. Particular disclosed embodiments of the method of using the signaling conjugate comprise multiplexing methods.

Signaling conjugates and methods of use

Disclosed herein are antibody-nanoparticle conjugates that include two or more nanoparticles (such as gold, palladium, platinum, silver, copper, nickel, cobalt, iridium, or an alloy of two or more thereof) directly linked to an antibody or fragment thereof through a metal-thiol bond. Methods of making the antibody-nanoparticle conjugates disclosed herein include reacting an arylphosphine-nanoparticle composite with a reduced antibody to produce an antibody-nanoparticle conjugate. Also disclosed herein are methods for detecting a target molecule in a sample that include using an antibody-nanoparticle conjugate (such as the antibody-nanoparticle conjugates described herein) and kits for detecting target molecules utilizing the methods disclosed herein.

Antibody-nanoparticle conjugates and methods for making and using such conjugates

Antibody/signal-generating moiety conjugates are disclosed that include an antibody covalently linked to a signal-generating moiety through a heterobifunctional polyalkyleneglycol linker. The disclosed conjugates show exceptional signal-generation in immunohistochemical and in situ hybridization assays on tissue sections and cytology samples. In one embodiment, enzyme-metallographic detection of nucleic acid sequences with hapten-labeled probes can be accomplished using the disclosed conjugates as a primary antibody without amplification.

Antibody conjugates

A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, athiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

Haptens, hapten conjugates, compositions thereof and method for their preparation and use

A method for performing a multiplexed diagnostic assay, such as for two or more different targets in a sample, is described. One embodiment comprised contacting the sample with two or more specific binding moieties that bind specifically to two or more different targets. The two or more specific binding moieties are conjugated to different haptens, and at least one of the haptens is an oxazole, a pyrazole, a thiazole, a nitroaryl compound other than dinitrophenyl, a benzofurazan, a triterpene, a urea, a thiourea, a rotenoid, a coumarin, a cyclolignan, a heterobiaryl, an azo aryl, or a benzodiazepine. The sample is contacted with two or more different anti-hapten antibodies that can be detected separately. The two or more different anti-hapten antibodies may be conjugated to different detectable labels.

Haptens, hapten conjugates, compositions thereof preparation and method for their preparation and use

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Tucson, AZ, United States of America

Jerome W Kosmeder, Ii