Pioneer Hi-bred International, Inc.

Other

Jerome P Ranch

Wallace A Marsh

William James Gordon-Kamm

Dwight T Tomes

Gary A Huffman

Rudolf Jung

Larry R Beach

Zuo-Yu Zhao

David S Ertl

Carl W Garnaat

A Gururaj Rao

Matthias Liebergesell

Locksley Earl McGann

Virginia M Dress

Regina K Higgins

John D Laurie

Guichang Zhang

David D Cass

Auxotrophicand methods employing auxotrophicare provided. Auxotrophicmay be used in a variety of methods including biologically containingcomprising a transgene and transforming a plant cell without using ancounter-selective agent. Transforming maize immature embryos using anauxotrophic for thymidine results in comparable transformation efficiency as transformation achieved using prototrophic. Methods for producing anthymidine auxotroph and using the auxotroph in transformation methods are disclosed. Transformed tissues and plants produced using methods of the present invention are also provided.

[object Object]

Methods are provided for transforming freshly isolated, immature maize embryos and for producing transgenic maize plants. The methods comprise obtaining immature embryos from a maize plant, contacting the embryos with an auxin-depleted or phytohormone-depleted transformation support medium and introducing a nucleotide construct into cells from the embryos prior to subjecting the embryos to conditions which promote embryogenic-tissue formation. The methods additionally comprise identifying or selecting transformed cells and regenerating such cells into transformed maize plants.

Methods for transforming immature maize embryos

The present invention provides a plant seed the endosperm of which is characterized as having an elevated level of a preselected amino acid. The present invention also provides expression cassettes, vectors, plants, plant cells and a method for enhancing the nutritional value of seeds.

Alteration of amino acid compositions in seeds

Methods for plant transformation, for improving transformation efficiency, and for producing transgenic plants are provided. The methods comprise crossing a recipient plant from a genetic line of a plant species of interest with a donor plant selected from a transformation competent genetic line of the same plant species or of another closely related plant species to obtain a hybrid plant. Tissues obtained from the hybrid plant are transformation competent. These tissues can then be transformed with one or more nucleotide sequences of interest and selected for transgenic events having the nucleotide sequence of interest integrated within a chromosome derived from the recipient plant. Transformed cells can be selected and transgenic hybrid plants regenerated. The nucleotide sequence of interest can be introgressed into the genetic line from which the original recipient parent was derived, or into other genetic lines. Transformed plants and seeds are additionally provided.

Methods of transforming plants and identifying parental origin of a chromosome in those plants

Methodology is provided for the production of uniformly transformed plants capable of transmitting a foreign gene to progeny by sexual reproduction. A foreign gene is introduced into the zygote in an isolated embryo sac and a transformed plant is recovered. Alternatively, a foreign gene is introduced into an egg cell in an isolated embryo sac, the egg cell is fertilized with an isolated sperm cell and a transformed plant is recovered. Sperm cells may be transformed with a foreign gene, an egg cell in an isolated embryo sac is fertilized with the transformed sperm cells, or nuclei isolated from the transformed sperm cells, and a transgenic plant is recovered. Another method for the production of transgenic plants is transformation of an embryo in an isolated embryo sac. The transgenic plant produced by any one of these methods is homogeneously transformed and capable of transmitting the foreign gene to progeny by sexual reproduction.

Transformation of zygote, egg or sperm cells and recovery of transformed plants from isolated embryo sacs

Growing community of inventors

West Des Moines, IA, United States of America

Jerome P Ranch