Harvard College

Medical Research Council

United Kingdom Research and Innovation

Capsum

Centre National De La Recherche Scientifique

Other

Commissariat &#xc0; L'&#xc9;nergie Atomique Et Aux &#xc9;nergies Alternatives

Rhodia Chimie

Université Pierre Et Marie Curie (Paris 6)

Université Paris Diderot-Paris 7

Horiba Abx Sas

National Science Foundation

Jerome Bibette

Darren Roy Link

Andrew David Griffiths

David A Weitz

Keunho Ahn

Mathieu Goutayer

Nicolas Bremond

Mikel Morvan

Gilles Guerin

Isabelle Texier-Nogues

Gilles Cauet

Yan Eric Pafumi

Philippe Nerin

Laurent Boitard

Fabrice Navarro Y Garcia

Sebastien Remi Bardon

Jean Baudry

Aurelien Daynes

Thomas Delmas

Ahmed Marouf

Nicolas Atrux-Tallau

Jean-Philippe Gineys

Hugo Domejean

Anette Funfak

Julien Sylvestre

Pierre Nassoy

Veronique Mourier-Robert

Thomas Mason

Kevin Alessandri

David Weitz

Kuenho Ahn

The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.

Compartmentalised combinatorial chemistry by microfluidic control

The invention describes a method for isolating one or more genetic elements encoding a gene product having a desired activity, comprising the steps of: (a) compartmentalising genetic elements into microcapsules; and (b) sorting the genetic elements which express the gene product having the desired activity; wherein at least one step is under microfluidic control. The invention enables the in vitro evolution of nucleic acids and proteins by repeated mutagenesis and iterative applications of the method of the invention.

Vitro evolution in microfluidic systems

The invention deals with a dispersion of drops of a first phase in a second phase substantially immiscible with the first phase, the solubility of the first phase in the second phase being less than 5% by mass. Each drop includes a core formed with a first phase and a shell formed with a coacervate layer interposed between the first phase and the second phase. The coacervate layer comprises a first precursor polymer of the coacervate and a second precursor polymer of the coacervate, one of the first phase and of the second phase is aqueous, and the other of the first phase and of the second phase is oily.

Dispersion of drops of a first phase, dispersed in a second phase substantially immiscible with the first phase

A capsule containing at least one mammalian cell, includes a liquid core, and at least one external envelope totally encapsulating the liquid core at its periphery, the external envelope including at least one gelled polyelectrolyte and/or a stiffened biopolymer and being able to retain the liquid core when the capsule is immersed in a gas. The present invention further relates to the method for preparing such a capsule, to a method for screening cosmetic active ingredients as well as a culture method using such capsules.

Capsules containing mammalian cells

The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalising the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.

Compartmentalised screening by microfluidic control

In vitro evolution in microfluidic systems

The invention describes a method for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, comprising the steps of: a) compartmentalizing the compounds into microcapsules together with the target, such that only a subset of the repertoire is represented in multiple copies in any one microcapsule; and b) identifying the compound which binds to or modulates the activity of the target; wherein at least one step is performed under microfluidic control. The invention enables the screening of large repertoires of molecules which can serve as leads for drug development.

This method includes the provision of a first fluid () including the first phase and a first precursor polymer of the coacervate contained in the first phase, and the formation of drops of the first fluid () in a second fluid () intended to form the second phase (). It includes the steps of introducing a second precursor polymer of the coacervate into the second fluid () and, during the formation of each drop (), or once each drop () has been formed, for generating the coacervate layer by interaction between the first precursor polymer and the second precursor polymer at the interface between the first phase () and the second phase ().

Method for forming drops of a first phase, dispersed in a second phase substantially immiscible with the first phase

The present invention relates to a flow assay method in a liquid medium for an object (or element) of interest via the formation of aggregates of particles that are surface-functionalized by at least one functionalizing molecule, or receptor, specific for said object of interest.

Flow assay method for an object of interest

The present invention relates to a therapeutic agent formulation in the form of a nano-emulsion, comprising a continuous aqueous phase and at least one dispersed oily phase, in which the oily phase comprises further to the therapeutic agent, at least one amphiphilic lipid and at least one solubilizing lipid, and in which the aqueous phase comprises at least one polyalkoxylated cosurfactant. The invention also relates to a preparation method and use of this formulation.

Encapsulation of lipophilic or amphiphilic therapeutic agents in nano-emulsion

The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalizing two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalized into the microcapsules.

The invention describes a method for the synthesis of compounds comprising the steps of: (a) compartmentalising two or more sets of primary compounds into microcapsules; such that a proportion of the microcapsules contains two or more compounds; and (b) forming secondary compounds in the microcapsules by chemical reactions between primary compounds from different sets; wherein one or both of steps (a) and (b) is performed under microfluidic control; preferably electronic microfluidic control, The invention further allows for the identification of compounds which bind to a target component of a biochemical system or modulate the activity of the target, and which is co-compartmentalised into the microcapsules.

The present invention relates to a method for preparing capsules comprising a liquid core, a stiffened intermediate envelope and a gelled external envelope, comprising a step of forming a multi-component liquid drop, a gelification step and a stiffening step. The present invention also relates to a method for preparing capsules comprising a liquid core and a stiffened envelope, comprising a step of forming a multi-component liquid drop, a gelification step, a stiffening step and a depolymerizing step.

Method for preparing stiffened capsules

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Paris, France

Jerome Bibette