Commonwealth Scientific and Industrial Research Organisation

Cook Medical Technologies LLC

Polynovo Biomaterials Pty Limited

Evonik Operations Gmbh

Jerome Anthony Werkmeister

John Alan Maurice Ramshaw

Alan George Brownlee

Christopher Malcolm Elvin

Charles Mark Lindall

Yong Yi Peng

Pathiraja Arachchillage Gunatillake

Raju Adhikari

Roshan Tyrrel Anton Mayadunne

Heng Chy Taing

Paul Richard Vaughan

Tam Phuong Thi Le

Jacinta Frances White

Violet Stoichevska

Tracy Ann Tebb

Maria Galanis

Veronica Glattauer

A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a cross-linking reaction to form a 3-dimensional matrix of the biomaterial.

Photoactivated crosslinking of a protein or peptide

The present disclosure relates to recombinant or synthetic collagen-like proteins comprising at least one triple-helical domain and wherein the collagen-like protein is modified compared to a native bacterial collagen-like sequence.

Modified bacterial collagen-like proteins

The present invention relates to a method for purifying triple-helical or collagen-like proteins recombinantly produced from a bacterial, yeast or plant host cell.

Purification of triple helical proteins

A method of crosslinking a protein or peptide for use as a biomaterial, the method comprising the step of irradiating a photoactivatable metal-ligand complex and an electron acceptor in the presence of the protein or peptide, thereby initiating a crosslinking reaction to form a 3-dimensional matrix of the biomaterial.

The present invention provides a biocompatible prepolymer comprising hydrophilic and hydrophobic segments, wherein the hydrophobic segments have at least one ethylenically unsaturated functional group and at least 5% of the segments have two or more ethylenically unsaturated functional groups and water. The invention further provides a biocompatible prepolymer composition comprising hydrophilic and hydrophobic prepolymers, wherein at least one of the hydrophobic prepolymers has at least one ethylenically unsaturated functional group and at least 5% of the prepolymers have two or more ethylenically unsaturated functional groups and water. The invention further provides use of the prepolymer or prepolymer compositions of the invention in biomedical applications such as tissue engineering, as bone substitutes or scaffolds, and in wound treatment.

Biocompatible polymer compositions

A method for producing a hydroxylated triple helical protein is disclosed, as well as a yeast host cell used in the method. The method comprises culturing a yeast host cell comprising: (1) a first DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, (2) a second DNA molecule comprising a DNA sequence encoding P4H&agr; subunit operably linked to a promoter functional in the yeast host cell, and (3) a third DNA molecule comprising a DNA sequence encoding a polypeptide or peptide operably linked to a promoter functional in the yeast host cell, wherein the polypeptide or peptide is one which, when hydroxylated, forms the hydroxylated triple helical protein; and wherein during culturing, each of the first DNA molecule, the second DNA molecule and the third DNA molecule are replicated, stably retained and segregated.

Method for producing, in yeast, a hydroxylated triple helical protein, and yeast host cells useful in said method

Growing community of inventors

Camberwell, Australia

Jerome Anthony Werkmeister