Advpharma, Inc.

Academia Sinica

National Taiwan University

National Health Research Institutes

Jeremy J W Chen

Pan-Chyr Yang

Tse-Ming Hong

Konan Peck

Cheng-Wen Wu

Yi-Jen Lee

Cheng-Chung Wu

Jin-Yuan Shin

Reen Wu

Sung-Liang Yu

Hsuan-Yu Chen

Shuenn-Chen Yang

Harn-Jing Terng

Fu Sheng Chang

Jin-Yuan Shih

Yeh-Tze Chou

Yi-Wen Chu

Methods for determining a tumor in a human is disclosed. Also disclosed are methods for identifying adenocarcinoma, and methods for identifying squamous cell carcinoma in a human tumor sample. In addition, methods for predicting prognosis of metastasis and survival in a human having a tumor is disclosed.

Metastasis-associated gene profiling for identification of tumor tissue, subtyping, and prediction of prognosis of patients

This invention provides a transcription unit which is isolated from the upstream nucleic acid sequence of the collapsing response mediator protein-1 (CRMP-1) gene, an invasion-suppressor gene. The transcription unit contains a nucleic acid regulatory sequence which demonstrates promoter and/or regulatory activities (such as providing a transcription factor binding site) to enhance the expression of the CRMP-1 and/or a reporter protein. The invention also provides a DNA construct containing this transcription unit which can be transfected into a host cell. Additionally, the invention provides methods to enhance the expression of CRMP-1 and/or the reporter protein. The over-expression of CRMP-1 in a cancer cell can inhibit the metastasis of the cancer cell.

Collapsin response mediator protein-1 (CRMP-1) transcriptional regulatory nucleic acid sequences

This invention is based on the discovery of an association of Collapsin Response Mediator Protein-1 (CRMP-1) with tumor metastasis. The level of CRMP-1 protein or mRNA can be used as an indicator of cellular invasiveness and of a test compound's ability to alter cellular invasiveness. The level of CRMP-1 protein can also be altered, e.g., to reduce invasiveness.

Collapsin response mediator protein-1

The invention relates to a method of detecting a differentially expressed gene in a first sample of nucleic acids representing a first population of RNA transcripts and a second sample of nucleic acids representing a second population of RNA transcripts. The nucleic acids in the samples are labled with a member of specific binding pair, and the labeled nucleic acids in each sample are then hybridized to an excess of copies of a gene-specific sequence. The hybridized nucleic acids in each sample are further labeled by binding a second member of the specific binding pair to the first member, in which the second member has an activity to convert a chromogenic substrate into a chromogen. As a result of contacting the second member with the chromogenic substrate, the chromogenic substrate is converted into the chromogen. A difference in the amounts of chromogen produced from assaying the two samples indicate that the gene-specific sequence is differentially expressed in the samples.

Methods for detecting differentially expressed genes

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Fongyuan, Taiwan

Jeremy J W Chen