Life Technologies Corporation

Pacific Biosciences of California, Inc.

Jeremy Gray

Alexander J Mastroianni

Marc Glazer

Ronald L Cicero

Wolfgang Hinz

James A Ball

Phil Waggoner

Christina E Inman

Yufang Wang

David Cox

Scott Parker

Michael L Minto

Kimberly Gorrell

Stephen P Dudek

Annette Claire Grot

Dmitriy Gremyachinskiy

Adrian Nielsen Fehr

David Light

Natasha Popovich

Abraham Rosenbaum

Hua Yu

Collyn Seeger

Thang Pham

Stephen Wilson Turner

Jonas Korlach

James M Bustillo

Robin A Emig

Kevin Travers

Paul M Kenney

Roman Rozhkov

Joseph Koscinski

Lei Sun

Arunashree Bhamidipati

Yu-Chih Tsai

John S Eid

Fred C Christians

Benjamin A Flusberg

Prasanna Krishnan Thwar

Jordan Owens

Eric Olivares

Robert Sebra

Steven Lin

Gregory Kearns

Mohammad Alanjary

Ravi Dalal

Aj Chmura

Albert James Chmura

Philip Waggoner

Paul Kenney

Wofgang Hinz

Pham Thang

The present disclosure provides methods, compositions and kits as well as systems for manipulating nucleic acids, including implementing isothermal amplification, such as recombinase-polymerase amplification (RPA), of a nucleic acid template using a pre-seeded solid support. Provided are rapid and efficient methods for generating template nucleic acid molecules comprising specific nucleotide sequence bound to solid support. Such methods can be used, for example, in manipulating nucleic acids in preparation for analysis methods that utilize monoclonal populations of nucleic acids.

Methods and compositions for manipulating nucleic acids

A sensor component includes a sensor including a sensor surface and a reaction site in cooperation with the sensor and exposing the sensor surface. The reaction site including a reaction site surface. A surface agent is bound to the reaction site surface or the sensor surface. The surface agent includes a surface active functional group reactive with Bronsted base or Lewis acid functionality on the reaction site surface or the sensor surface and including distal functionality that does not have a donor electron pair.

Surface treatment of semiconductor sensors

A sensor apparatus includes a substrate, a semiconductor device disposed over the substrate, the semiconductor device having a surface electrode structure, and a saccharide coating formed over the surface electrode structure. The saccharide coating can be removed prior to use. The semiconductor device can further include a well and optionally a bead disposed in the well.

Surface stabilization of biosensors

A method of forming a polymer matrix array includes treating a surface within a well of a well array with a surface compound including a surface reactive functional group and a radical-forming distal group; applying an aqueous solution including polymer precursors to the well of the well array; and activating the radical-forming distal group of the surface coupling compound with an initiator and atom transfer radical polymerization (ATRP) catalyst to initiate radical polymerization of the polymer precursors within the well of the well array to form the polymer matrix array.

Matrix arrays and methods for making same

The application relates to methods of analyzing luminescent species. A substrate is provided that has a plurality of zero mode waveguides having apertures that extend through an upper non-reflective layer that is disposed on a lower transparent layer of a substrate. The apertures have non-reflective oxide layers on the reflective side walls of the apertures, the side walls having a thickness of greater than 10 nm, and the oxide layer is formed by oxidizing the non-reflective layer. The volume within the oxide layer defines a solution volume, and the volume within the reflective walls defines a ZMW volume. Having such non-reflective layers on the walls of the ZMW usefully decouples the solution volume from the ZMW volume.

Method for analyzing luminescent species

A method of forming a polymer matrix array includes applying an aqueous solution into wells of a well array. The aqueous solution includes polymer precursors. The method further includes applying an immiscible fluid over the well array to isolate the aqueous solution within the wells of the well array and polymerizing the polymer precursors isolated in the wells of the well array to form the polymer matrix array. An apparatus includes a sensor array, a well array corresponding to the sensor array, and an array of polymer matrices disposed in the well array.

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Larkspur, CA, United States of America

Jeremy Gray